Vascular endothelial growth factor 165 promotes proliferation of human adipose-derived mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2015.28.011

Abstract

Abstract:

BACKGROUND: Autologous fat transplantation has been widely used in soft tissue defect repair and cosmetic surgery, and the 1-year transplant survival rate is 20%-80%. Therefore, the establishment of timely and adequate blood supply at early period after transplantation is very important for the survival of transplanted fat tissues.

OBJECTIVE: To observe the proliferation of human adipose-derived mesenchymal stem cells transfected with vascular endothelial growth factor 165.

METHODS: Human adipose-derived mesenchymal stem cells were subcultured in vitro. Recombinant adenovirus carrying vascular endothelial growth factor 165 and blank virus were respectively transferred into adipose-derived mesenchymal stem cells. Cells cultured normally served as blank group.

RESULTS AND CONCLUSION: Compared with the control and blank groups, the expressions of vascular endothelial growth factor 165 mRNA and protein were higher in the experimental group (P < 0.05). Experimental findings suggest that the recombinant adenovirus carrying vascular endothelial growth factor 165 transferred into adipose-derived mesenchymal stem cells cannot only maintain the expression of target protein but also obviously promote the proliferation of adipose-derived mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Cell proliferation, Viruses

CLC Number:

R394.2

Wang Yu, Zhu Zhi-tu, Chen Jun-jiang. Vascular endothelial growth factor 165 promotes proliferation of human adipose-derived mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4485-4492.

Figures/Tables 6

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