Endothelial progenitor cells from human umbilical cord blood and peripheral blood: Isolation, culture and identification

doi:10.3969/j.issn.2095-4344.2013.14.016

Abstract

Abstract:

BACKGROUND: There are many articles on the isolation, culture and identification of endothelial progenitor cells at home and abroad, while the articles regarding the endothelial progenitor cells from the umbilical cord blood and peripheral blood are rare.
OBJECTIVE: To isolate, culture and identify the endothelial progenitor cells from umbilical cord blood and peripheral blood.
METHODS: Mononuclear cells were isolated from umbilical cord blood and peripheral blood with density gradient centrifugation method, and then the cells were implanted on the culture plate pre-paved with fibronectin with the concentration of 1×10⁶/cm². After that, the cells were induced and cultured with M199 medium containing vascular endothelial growth factor.
RESULTS AND CONCLUSION: The endothelial progenitor cells could be isolated and obtained from peripheral blood and umbilical cord blood, and the mononuclear cells obtained with density gradient centrifugation and adherence screening method could be differentiated into endothelial progenitor cells after induced with vascular endothelial growth factor. The endothelial progenitor cells could express CD34, CD133, CD105, KDR and CD13, could swallow acetylated low density lipoprotein and be combined with with ulex europaeus agglutinin 1, which could be considered as the sign of in vitro sorting of endothelial progenitor cells.

Key words: stem cells, stem cell culture and differentiation, endothelial progenitor cells, angiogenesis, new endothelium, human umbilical cord blood, peripheral blood, stem cells photographs-containing paper

CLC Number:

R394.2

Li Huan-huan, Zhao Li-jing, He Xu, Wang Juan, Liu Kang-ding. Endothelial progenitor cells from human umbilical cord blood and peripheral blood: Isolation, culture and identification[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(14): 2578-2585.

Figures/Tables 5

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