Effect of adipose stem cell transplantation on the neuromuscular regeneration unit of Duchenne muscular dystrophy mice

doi:10.3969/j.issn.2095-4344.2015.19.016

Abstract

Abstract:

BACKGROUND: Currently, there is no effective treatment for Duchenne muscular dystrophy. Previous studies have shown that gene therapy and stem cell transplantation therapy are possible to be “cure” approaches. Therefore, this study intended to evaluate their efficacy in animal models, and to verify the hypothesis of neuromuscular regeneration unit.
OBJECTIVE: To explore the efficacy and feasibility of adipose stem cell transplantation for Duchenne muscular dystrophy and to observe the effects of cell transplantation on muscle fibers, new vessels and nerve endings.
METHODS: Adipose stem cells from mdx mice were isolated and cultured in vitro, and then modified by recombinant baculovirus followed by implantation into mouse models of Duchenne muscular dystrophy. Serum creatine kinase level, muscle pathological changes and muscle dystrophin expression were detected in experimental animals after transplantation; immunofluorescence staining was used to detect vascular, muscle and nerve regeneration after cell transplantation.
RESULTS AND CONCLUSION: After transplantation, dystrophin expression was found that could reduce and
even reverse the pathological damage of muscles to a certain extent, thereby to reduce the serum level of creatine kinase. In addition, stem cells-derived muscle fiber, vasular endothelial cells and nerve ending were formed after cell transplantation. These evidences domenstrate that adipose stem cell transplantation is one of promising treatments for Duchenne muscular dystrophy.

Key words: Stem Cells, Adipose Tissue, Muscular Dystrophy, Duchenne

CLC Number:

R394.2

Kong Jie,, Cao Ji-qing, Yang Juan, Chen Fei, Li Ya-qin, Huang Ru-cheng, Jin Yuan-lin. Effect of adipose stem cell transplantation on the neuromuscular regeneration unit of Duchenne muscular dystrophy mice[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3042-3048.

Figures/Tables 4

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