Sequence analysis and identification of a novel allele, HLA-B*55:46

doi:10.3969/j.issn.2095-4344.2015.19.015

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (19): 3037-3041.doi: 10.3969/j.issn.2095-4344.2015.19.015

Previous Articles Next Articles

Sequence analysis and identification of a novel allele, HLA-B*55:46

Yin Hong¹, Li Xiao-feng^{2, 3, 4}, Li Jian-ping^{2, 3, 4, 5}

¹Department of Anaesthesia, Shengjing Hospital Affiliated to China Medical University, Shenyang 110041, Liaoning Province, China; ²Transfusion Medicine Institute, Liaoning Blood Center, Shenyang 110044, Liaoning Province, China; ³Key Laboratory of Blood Safety Research of Liaoning, Shenyang 110044, Liaoning Province, China; 4Key Laboratory of Blood Safety Research of Shenyang, Shenyang 110044, Liaoning Province, China; 5Shenyang Research Center for Stem Cell Clinical Application, Shenyang 110044, Liaoning Province, China

Online:2015-05-06 Published:2015-05-06
Contact: Li Jian-ping, M.D., Chief physician, Transfusion Medicine Institute, Liaoning Blood Center, Shenyang 110044, Liaoning Province, China; Key Laboratory of Blood Safety Research of Liaoning, Shenyang 110044, Liaoning Province, China; Key Laboratory of Blood Safety Research of Shenyang, Shenyang 110044, Liaoning Province, China; Shenyang Research Center for Stem Cell Clinical Application, Shenyang 110044, Liaoning Province, China
About author:Yin Hong, M.D., Department of Anaesthesia, Shengjing Hospital Affiliated to China Medical University, Shenyang 110041, Liaoning Province, China
Supported by:
Shenyang Science and Technology Project, No. F10-206-1-00

Abstract

Abstract:

BACKGROUND: In recent years, with the development of China Marrow Donor Program and the improvement of human leukocyte antigen (HLA) typing technique, novel HLA alleles have been discovered constantly in China.
OBJECTIVE: To identify and confirm a novel HLA allele in a Chinese individual.
METHODS: A new HLA allele was found in a 27-year-old male Han volunteer donor of hematopoietic stem cells during routine HLA genotyping by PCR-sequence specific oligonucleotide probes and sequencing-based typing.
RESULTS AND CONCLUSION:The HLA-B locus hybridization probe reaction patterns of this sample did not match to any known HLA-B alleles or allelic combinations. Exons 2-3 were sequenced in both directions using HLA-B sequence primer and group-specific sequencing primer. The new sequence had 1 nt change from its closest allele B*55:02:01 at nucleotide 412 where A→G (codon138 AAC→GAC) resulting in a coding change,
138 Asparagine (N) was changed to Aspartate (D). The nucleotide sequence has been submitted to the GenBank nucleotide sequence database and it is available under the accession number HM989018. A novel HLA allele, HLA-B*55:46, has been identified, and named officially by the WHO Nomenclature Committee.

Key words: 干细胞, 培养, 新等位基因, HLA-B*55:46, 序列分析, 聚合酶链式反应, 序列特异性寡核苷酸探针, 碱基, 氨基酸, 人类白细胞抗原, 基因座, 外显子

CLC Number:

R394.2

Yin Hong, Li Xiao-feng, Li Jian-ping. Sequence analysis and identification of a novel allele, HLA-B*55:46[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(19): 3037-3041.

Figures/Tables 2

References

[1] Robinson J, Malik A, Parham P, et al. IMGT/HLA database--a sequence database for the human major histocompatibility complex. Tissue Antigens. 2000;55(3):280-287.

[2] 李晓丰,章旭,张坤莲,等.中国人群新等位基因HLA-B*40:74的序列分析及认定[J].中华微生物学和免疫学杂志,2011,31(12): 1068-1071.

[3] 李晓丰,章旭,陈阳,等.人类白细胞抗原新等位基因HLA-A*3020的鉴定及确认[J].中华医学遗传学杂志,2010,27(1):96-99.

[4] 章旭,蒋术一,李晓丰,等.人类白细胞抗原新等位基因B*54:09的序列分析[J].中华医学遗传学杂志,2012,29(1):91-94.

[5] 陈阳,刘显智,李晓丰,等.新等位基因HLA-B*9526的确认和序列分析[J].中华医学遗传学杂志,2009,26(6):705-708.

[6] 程四国,张伯伟.HLA新等位基因A~*9217家系调查分析[J].中国组织工程研究与临床康复,2009,13(53):10537-10540.

[7] 王琳,宋长兴,张志欣.新等位基因人类白细胞抗原B*15:133的鉴定及其原核表达[J].中国组织工程研究与临床康复,2011,15(5): 878-881.

[8] 张毅,房云海,聂向民,等.HLA新等位基因B*4086的确认及家系调查[J].复旦学报(医学版),2009,36(6):707-709,714.

[9] 刘娜,单小燕,李伟,等.DNA测序鉴定新等位基因人白细胞抗原-Cw*1521[J]. 中华检验医学杂志,2009,32(1):71-72.

[10] 李晓丰,章旭,张坤莲,等.新等位基因HLA-B*37:04:02的序列分析[J].中华医学遗传学杂志,2014,31(4):515-517.

[11] 李晓丰,章旭,张坤莲,等.HLA-Ⅰ类基因新等位基因HLA-B*52:11的序列分析及确认[J].中华医学遗传学杂志,2011,28(6): 712-715.

[12] 李晓丰,章旭,张坤莲,等.HLA新等位基因HLA-B*08∶57的核苷酸序列分析[J].中华微生物学和免疫学杂志,2013,(11):827-827.

[13] Marsh SG; WHO Nomenclature Committee for Factors of the HLA System. Nomenclature for factors of the HLA system, update August 2010. Tissue Antigens. 2011;77(2):167-175.

[14] 张志欣.HLA新等位基因的确认与研究及其在我国的开展[J].中国输血杂志,2008,21(2):81-83.

[15] Bjorkman PJ, Saper MA, Samraoui B, et al. The foreign antigen binding site and T cell recognition regions of class I histocompatibility antigens. Nature. 1987;329(6139):512-518.

[16] 李晓丰, 李剑平,迟立平,等.流式磁珠反向杂交法HLA基因分型中磁珠读数减少对结果的影响[J].中国输血杂志,2007,20(1): 37-39.

[17] Cesbron-Gautier A, Simon P, Achard L, et al. Luminex technology for HLA typing by PCR-SSO and identification of HLA antibody specificities. Ann Biol Clin (Paris). 2004;62(1): 93-98.

[18] 鞠瑞青,陈琳,林乾飞,等.HLA 新等位基因HLA-B*5145 的确认[J].中国组织工程研究与临床康复,2010,14(44):8249-8252.

[19] Zhu F, He Y, Zhang W, et al. Analysis for complete genomic sequence of HLA-B and HLA-C alleles in the Chinese Han population. Int J Immunogenet. 2011;38(4):281-284.

[20] Zhang KL, Li JP, Zhang X, et al. A novel HLA-B*46 allele, B*4609, identified by sequence-based typing in a Chinese donor. Tissue Antigens. 2007;70(4):344-345.

[21] Xu YP1, Deng ZH, Zou HY, et al. Cloning and sequencing HLA-A and -B genomic DNA and analyzing polymorphism in regulatory regions in Chinese Han individuals. Yi Chuan. 2010;32(7):685-693.

[22] 王大明,何柳媚,邹红岩,等.4 例人类白细胞抗原罕见等位基因的结果分析[J].中国组织工程研究,2013,17(44):7809-7814.

[23] 胡彬,迟晓云,冯智慧,等.新等位基因HLA-A*11：97与HLA-B*44：127的确认[J].中国组织工程研究,2012,16(53):9999-10004.

[24] 戚新,李归冀,章旭,等.人类白细胞抗原新等位基因HLA-A*31：22的序列分析及确认[J].中华微生物学和免疫学杂志,2012,32(12): 1011-1014.

[25] Liu XZ, Li XF, Zhang X, et al. A new human leukocyte antigen-B allele, HLA-B*52:11. Tissue Antigens. 2011;77(3): 261-262.

[26] Li JP, Li XF, Chen Y, et al. Identification of a new human leukocyte antigen A allele, HLA-A*3020. Tissue Antigens. 2009;73(6):606-607.

[27] 周丹.中国汉族人群人类白细胞抗原新等位基因A*k1155的确认[J].中国组织工程研究与临床康复,2010,14(44):8241-8244.

[28] 吴国光,邓志辉,高素青,等.6965名汉族骨髓供者HLA多态性分析[J].中华血液学杂志,2004,25(8):473-477.

[29] 张洪波,赖江华,李生斌.西北地区汉族人群HLA-A、-B、-DRB1基因座单倍型分析[J].中华医学遗传学杂志,2005, 22(4): 464-466.

[30] 陈阳,李剑平.辽宁汉族人群HLA-B等位基因多态性的分布[J].中华医学遗传学杂志,2006,23(4):461-462.

[31] 蒋雪梅,陈士俊,旭日.山东汉族人乙肝患者与HLA-B基因型的相关性研究[J].山东大学学报(医学版),2005,43(8):737-740.

[32] 肖露露,于立新,陈洪涛,等.人类免疫遗传基因在慢性肾小球肾炎病人中的表达[J].广州医药,1999,30(5):8-10.

[33] 周月,李剑平. 人类白细胞抗原新等位基因B*07:110的序列分析及确认[J]. 中国组织工程研究,2015,19(1):135-139.

Sequence analysis and identification of a novel allele, HLA-B*55:46

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 2

References

Related Articles 7

Recommended Articles

Metrics

Comments

[1]	Sun Tianjing , Liu Sijia , Xie Fangke , Huang Xiaofei , Zhang Ji , Jiang Xuheng , Feng Hua, Yu Anyong. Role and hotspots of stem cell-derived exosome in the repair of traumatic brain injury [J]. Chinese Journal of Tissue Engineering Research, 2021, 25(1): 123-127.
[2]	Bai Dong, Zhou Zhong-xiao, Zhang Jian. Hepatocyte proliferation and apoptosis under regulation of human telomerase reverse transcriptase gene-modified bone marrow mesenchymal stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5118-5122.
[3]	Yang Chun-yan, Xu Ping, Li Bao-yun, Yang Yu-jun, Jia Huan-rong, Zhou Li-ying, Yang Qiao-zhi. Autologous umbilical cord blood mononuclear cell transfusion in preterm children: immune function and prognosis [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(28): 4572-4575.
[4]	He Ze-bin, Zhao Yun-he, Yang Gui-jiao, Lu Li. Effect of p53 inhibitor on viability of human bone marrow mesenchymal stem cells in late-phase amplification [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3616-3620.
[5]	Shao Shuai, Zhou Chen-hong, Xu Li-li. Differentiation of bone marrow and umbilical cord blood mesenchymal stem cells into osteoblasts in vitro: comparison of their osteogenic potentials [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3652-3657.
[6]	Zhao Zhen-de. Artificial bone combined with bone marrow mesenchymal stem cells for repairing femoral head necrosis in rabbits [J]. Chinese Journal of Tissue Engineering Research, 2012, 16(38): 7057-7062.
[7]	Yang Wen, Qin Shan-yu, Jiang Hai-xing, Zhang Jun-hong, Ning Lin, Meng Yun-chao. 骨髓间充质干细胞体外调控肝星状细胞死亡受体5的表达 [J]. Chinese Journal of Tissue Engineering Research, 2012, 16(27): 4947-4952.