Differentiation of bone marrow and umbilical cord blood mesenchymal stem cells into osteoblasts in vitro: comparison of their osteogenic potentials

doi:10.3969/j.issn.2095-4344.2015.23.008

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cells isolated from cord blood and bone marrow have multi-directional differentiation ability under a certain condition of induction.
OBJECTIVE: To compare the difference of differentiation of umbilical cord blood and bone marrow mesenchymal stem cells into osteoblasts.
METHODS: Human umbilical cord blood and bone marrow mesenchymal stem cells were isolated and cultured by density gradient method. When reached 90% confluency, mesenchymal stem cells were digested by trypsin for subculture. At the third passage, umbilical cord blood mesenchymal stem cells and bone marrow mesenchymal stem cells at 8×10⁴/well were incubated. When reached 80% confluency, cells were treated with low-glucose DMEM supplemented with 10% fetal bovine serum, 0.1 μmol/L dexamethasone, 50 μmol/L vitamin C and 10 mmol/L β-sodium glycerophosphate.
RESULTS AND CONCLUSION: There was no significant difference in morphology and biological properties of the two kinds of mesenchymal stem cells. Cells were highly expressed CD44, CD29, but did not express CD34. They had the ability to differentiate into osteoblasts, which had a positive staining for known markers: alkaline phospatase and calcium in vitro mineralization. There was no significant difference in the activity of osteoblasts of two kinds of cells. Results verify that umbilical cord blood and adult bone marrow mesenchymal stem cells can be induced into osteoblasts with a similar ability, and they can be used as seed cells for bone tissue engineering.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: 干细胞, 脐带脐血干细胞, 脐血间充质干细胞, 骨髓间充质干细胞, 成骨细胞, 诱导分化

CLC Number:

R394.2

Shao Shuai, Zhou Chen-hong, Xu Li-li. Differentiation of bone marrow and umbilical cord blood mesenchymal stem cells into osteoblasts in vitro: comparison of their osteogenic potentials [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3652-3657.

Figures/Tables 4

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