Effects of chiral polyethyleneimine/graphene oxide/phenylalanine interface on adhesion and proliferation of multilineage-differentiating stress enduring cells

doi:10.12307/2023.305

Abstract

Abstract: BACKGROUND: In recent years, multilineage-differentiating stress enduring (Muse) cell has attracted increasing attention due to its pluripotent differentiation capacity and non-tumorigenicity. Cell therapy based on Muse cells has shown exciting results in the animal experiments of many diseases. However, Muse cells face the difficulties of slow proliferation in long-term in vitro culture.
OBJECTIVE: To investigate the effect of chirals interfaces on adhesion and proliferation of Muse cells.
METHODS: The polyethyleneimine/graphene oxide/phenylalanine chiral biointerfaces (PEI/GO/L-Phe and PEI/GO/D-Phe) were constructed based on the layer-by-layer self-assembly method using polyethyleneimine (PEI) as the substrate layer and graphene oxide (GO) and L/D-phenylalanine (L/D-Phe) sequentially deposited. The effect of self-assembled chiral biointerfaces on the adhesion and proliferation behavior of Muse cells was investigated.
RESULTS AND CONCLUSION: (1) PEI/GO/L-Phe and PEI/GO/D-Phe chiral interfaces were successfully constructed based on facile self-assembly technology. (2) The statistical results showed that the chirality on the interface influenced Muse cell behaviors significantly. The initial adhesion and subsequent proliferation of Muse cells were enhanced by the PEI/GO/L-Phe interface, probably owing to the preferred adsorption of proteins, which further promote Muse cell proliferation.

Key words: regenerative medicine, Muse cells, chiral interfaces, layer self-assembly, phenylalanine enantiomers, adhesion, proliferation, protein adsorption

CLC Number:

Zhou Jingjing, Sun Yixin, Sheng Yang, Zhang Rong. Effects of chiral polyethyleneimine/graphene oxide/phenylalanine interface on adhesion and proliferation of multilineage-differentiating stress enduring cells[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(10): 1547-1552.

Figures/Tables 8

References

[1] LANGER R, TIRRELL DA. Designing materials for biology and medicine. Nature. 2004;428(6982):487-492.
[2] HIGUCHI A, LING QD, CHANG Y, et al. Physical cues of biomaterials guide stem cell differentiation fate. Chem Rev. 2013;113(5):3297-3328.
[3] 郑颖,邓诗碧,陈方.干细胞与再生医学技术发展态势研究[J].中国生物工程杂志,2022,42(4):111-119.
[4] MAO AS, MOONEY DJ. Regenerative medicine: Current therapies and future directions. Proc Natl Acad Sci U S A. 2015;112(47):14452-14459.
[5] SPILLER KL, KOH TJ. Macrophage-based therapeutic strategies in regenerative medicine. Adv Drug Deliv Rev. 2017;122:74-83.
[6] KESHTKAR S, AZARPIRA N, GHAHREMANI MH. Mesenchymal stem cell-derived extracellular vesicles: novel frontiers in regenerative medicine. Stem Cell Res Ther. 2018;9(1):63.
[7] OGURA F, WAKAO S, KURODA Y, et al. Human adipose tissue possesses a unique population of pluripotent stem cells with nontumorigenic and low telomerase activities: potential implications in regenerative medicine. Stem Cells Dev. 2014;23(7):717-728.
[8] KURODA Y, KITADA M, WAKAO S, et al. Unique multipotent cells in adult human mesenchymal cell populations. Proc Natl Acad Sci U S A. 2010;107(19):8639-8643.
[9] WAKAO S, AKASHI H, KUSHIDA Y, et al. Muse cells, newly found non-tumorigenic pluripotent stem cells, reside in human mesenchymal tissues. Pathol Int. 2014;64(1):1-9.
[10] FISCH SC, GIMENO ML, PHAN JD, et al. Pluripotent nontumorigenic multilineage differentiating stress enduring cells (Muse cells): a seven-year retrospective. Stem Cell Res Ther. 2017;8(1):227.
[11] KURODA Y, WAKAO S, KITADA M, et al. Isolation, culture and evaluation of multilineage-differentiating stress-enduring (Muse) cells. Nat Protoc. 2013;8(7):1391-1415.
[12] YAMASHITA T, KUSHIDA Y, WAKAO S, et al. Therapeutic benefit of Muse cells in a mouse model of amyotrophic lateral sclerosis. Sci Rep. 2020; 10(1):17102.
[13] NODA T, NISHIGAKI K, MINATOGUCHI S. Safety and Efficacy of Human Muse Cell-Based Product for Acute Myocardial Infarction in a First-in-Human Trial. Circ J. 2020;84(7):1189-1192.
[14] YAMADA Y, MINATOGUCHI S, KANAMORI H, et al. Stem cell therapy for acute myocardial infarction - focusing on the comparison between Muse cells and mesenchymal stem cells. J Cardiol. 2022;80(1):80-87.
[15] TAKAGI T, YOSHIMURA S, SAKUMA R, et al. Novel Regenerative Therapies Based on Regionally Induced Multipotent Stem Cells in Post-Stroke Brains: Their Origin, Characterization, and Perspective. Transl Stroke Res. 2017;8(6):515-528.
[16] YU J, HU K, SMUGA-OTTO K, et al. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009;324(5928): 797-801.
[17] WAKAO S, KITADA M, KURODA Y, et al. Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages. Methods Mol Biol. 2012;826:89-102.
[18] WANG L, XIAO L, ZHANG RZ, et al. Effects of acrylate/acrylamide polymers on the adhesion, growth and differentiation of Muse cells. Biomed Mater. 2018;14(1):015003.
[19] 李秀亚,盛扬,王圣依,等.蛋白芯片筛选用于培养Muse细胞的纤维蛋白原和明胶组合基质[J].中国组织工程研究,2022,26(21): 3312-3318.
[20] TRAPPMANN B, GAUTROT JE, CONNELLY JT, et al. Extracellular-matrix tethering regulates stem-cell fate. Nat Mater. 2012;11(7):642-649.
[21] MAMMOTO A, MAMMOTO T, KANAPATHIPILLAI M, et al. Control of lung vascular permeability and endotoxin-induced pulmonary oedema by changes in extracellular matrix mechanics. Nat Commun. 2013;4:1759.
[22] GENTILE F, MEDDA R, CHENG L, et al. Selective modulation of cell response on engineered fractal silicon substrates. Sci Rep. 2013;3:1461.
[23] WANG X, GAN H, ZHANG M, et al. Modulating cell behaviors on chiral polymer brush films with different hydrophobic side groups. Langmuir. 2012;28(5):2791-2798.
[24] QUAH SP, ZHANG Y, FLUERASU A, et al. Techniques to characterize dynamics in biomaterials microenvironments: XPCS and microrheology of alginate/PEO-PPO-PEO hydrogels. Soft Matter. 2021;17(6):1685-1691.
[25] ZHOU Q, GE L, GUIMARÃES CF, et al. Development of a Novel Orthogonal Double Gradient for High-Throughput Screening of Mesenchymal Stem Cells-Materials Interaction. Advanced Materials Interfaces. 2018;5(18):1800504-1800511.
[26] ABARICIA JO, FARZAD N, HEATH TJ, et al. Control of innate immune response by biomaterial surface topography, energy, and stiffness. Acta Biomater. 2021;133:58-73.
[27] SOTO VELIZ D, KUMMALA R, ABITBOL T, et al. Influence of mineral coatings on fibroblast behaviour: The importance of coating formulation and experimental design. Colloids Surf B Biointerfaces. 2021;208:112059.
[28] JOSEPH G, ORME RP, KYRIACOU T, et al. Effects of Surface Chemistry Interaction on Primary Neural Stem Cell Neurosphere Responses. ACS Omega. 2021;6(30):19901-19910.
[29] BAI CL, LIU MH. Implantation of nanomaterials and nanostructures on surface and their applications. Nano Today. 2012;7(4):258-281.
[30] Mei Y, Saha K, Bogatyrev SR, et al. Combinatorial development of biomaterials for clonal growth of human pluripotent stem cells. Nat Mater. 2010;9(9):768-778.
[31] SUN T, HAN D, RIEHEMANN K, et al. Stereospecific interaction between immune cells and chiral surfaces. J Am Chem Soc. 2007;129(6):1496-1497.
[32] YAO X, HU Y, CAO B, et al. Effects of surface molecular chirality on adhesion and differentiation of stem cells. Biomaterials. 2013;34(36): 9001-9009.
[33] LIU GF, ZHANG D, FENG CL. Control of three-dimensional cell adhesion by the chirality of nanofibers in hydrogels. Angew Chem Int Ed Engl. 2014;53(30):7789-7793.
[34] HU B, DENG J, ZHENG H, et al. Synthesis of Chiral Oligomer-Grafted Biodegradable Polyurethanes and Their Chiral-Dependent Influence on Bone Marrow Stem Cell Behaviors. Macromol Rapid Commun. 2016;37(16):1331-1336.
[35] DENG J, ZHENG HH, ZHENG XW, et al. Gold nanoparticles with surface-anchored chiral poly(acryloyl-L(D)-valine) induce differential response on mesenchymal stem cell osteogenesis. Nano Research. 2016;9(12): 3683-3694.
[36] PICCININI E, BLIEM C, REINER-ROZMAN C, et al. Enzyme-polyelectrolyte multilayer assemblies on reduced graphene oxide field-effect transistors for biosensing applications. Biosens Bioelectron. 2017;92:661-667.
[37] KWON D, KIM JS, CHA BH, et al. The Effect of Fetal Bovine Serum (FBS) on Efficacy of Cellular Reprogramming for Induced Pluripotent Stem Cell (iPSC) Generation. Cell Transplant. 2016;25(6):1025-1042.
[38] WANG X, GAN H, SUN T. Chiral Design for Polymeric Biointerface: The Influence of Surface Chirality on Protein Adsorption. Adv Funct Mater. 2011;21(17):3276-3281.
[39] OKUTAN M, DELIGOZ H. Effect of external salt addition on the structural, morphological and electrochemical properties of flexible PEDOT:PSS based LbL multilayered films. Colloids Surf A Physicochem Eng Asp. 2019;580(5):123695-123706.
[40] MAHERALI N, SRIDHARAN R, XIE W, et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell. 2007;1(1):55-70.
[41] SUKHIKH GT, MALAITSEV VV, BOGDANOVA IM, et al. Mesenchymal stem cells. Bull Exp Biol Med. 2002;133(2):103-109.
[42] HAN H, WANG K, FAN Y, et al. Enhancement of nitric oxide release and hemocompatibility by surface chirality of D-tartaric acid grafting. Applied Surface Science. 2017;425(15):847-854.
[43] EITAN E, ZHANG S, WITWER KW, et al. Extracellular vesicle-depleted fetal bovine and human sera have reduced capacity to support cell growth. J Extracell Vesicles. 2015;4:26373.
[44] VOGEL V, SHEETZ M. Local force and geometry sensing regulate cell functions. Nat Rev Mol Cell Biol. 2006;7(4):265-275.