Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (10): 1534-1540.doi: 10.12307/2023.289
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Chen Jiana1, 2, Nie Minhai1, 2, Hu Xinyue1, 2, Liu Xuqian1, 2
Received:
2022-01-15
Accepted:
2022-05-21
Online:
2023-04-08
Published:
2022-09-08
Contact:
Liu Xuqian, MD, Associate professor, Department of Periodontics & Oral Mucosal Diseases, Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, Sichuan Province, China
About author:
Chen Jiana, Master candidate, Department of Periodontics & Oral Mucosal Diseases, Affiliated Stomatology Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Luzhou Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Southwest Medical University, Luzhou 646000, Sichuan Province, China
Supported by:
CLC Number:
Chen Jiana, Nie Minhai, Hu Xinyue, Liu Xuqian. Platelet-derived growth factor BB promotes proliferation and osteogenic differentiation of human periodontal ligament stem cells[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(10): 1534-1540.
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2.5 矿化实验结果 第3代人牙周膜干细胞培养3 d后,在显微镜下观察细胞形态类似于成纤维细胞,大多呈梭形,有些呈多角形,形状不规则,其排列具有明显的方向性;加入成骨诱导液成骨诱导7 d后,成骨诱导液+ PDGF-BB组和成骨诱导液组细胞逐渐变为方形,出现灰白色钙化小结节、钙盐沉积和矿化结节,成骨诱导液+PDGF-BB组的钙结节与成骨诱导液组无明显差异,空白对照组无钙化结节形成;成骨诱导14 d时,成骨诱导液+PDGF-BB组镜下钙化结节多为板层状的圆形或椭圆形团块且多于成骨诱导液组,空白对照组无钙化结节形成;成骨诱导21 d,成骨诱导液组和成骨诱导液+ PDGF-BB镜下观察均可见大量钙化结节,但各组与14 d相比无明显差异,空白对照组无钙化结节形成,见图7。"
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