Radial extracorporeal shock wave therapy promotes the proliferation of neural stem cells in hippocampus of cerebral infarction rats and inhibits miR-124 expression

doi:10.12307/2021.140

Abstract

Abstract: BACKGROUND: miR-124 plays an important role in the proliferation of neural stem cells and changes in brain tissue and blood expression after cerebral infarction, suggesting that it is one of the core regulators of endogenous nerve regeneration after cerebral infarction. Radial extracorporeal shock wave therapy can promote neural stem cell proliferation, but whether it is related to miR-124 is currently unclear.
OBJECTIVE: To investigate the effect of radial extracorporeal shock wave therapy on the expression of miR-124 in the hippocampus after cerebral infarction and whether the promotion of neural stem cell proliferation is related to miR-124.
METHODS: A model of middle cerebral artery occlusion in rats was established. Rats were randomly divided into radial extracorporeal shock wave group and control group. The radial extracorporeal shock wave group was subjected to radial extracorporeal shock wave therapy intervention, with the ischemic head 72 hours after the cerebral infarction, once every 3 days. At 11, 20, 29, and 38 days after model establishment, modified neurological function scores were assessed in rats. Then RT-qPCR was used to detect the expressions of miR-124 and Nestin mRNA in the hippocampus of cerebral infarction rats. Western blot assay and immunofluorescence were used to detect the expression of Nestin protein in the hippocampus of the ischemic side.
RESULTS AND CONCLUSION: (1) After cerebral infarction in rats, the expression of miR-124 gradually increased in the hippocampus of the ischemic side. (2) At 11, 20, 29, and 38 days after middle cerebral artery occlusion, the neurological scores of the rats were lower in the radial extracorporeal shock wave group than those in the control group (P < 0.05). The expression of Nestin was higher on the ischemic side of the hippocampus than that in the control group (P < 0.05). (3) At 11, 20 and 29 days after middle cerebral artery occlusion, the expression of miR-124 in hippocampus of cerebral infarction rats on the affected side in the radial extracorporeal shock wave group was lower than that of the control group (P < 0.05). (4) Results suggest that expression of miR-124 in the hippocampus of cerebral infarction rats is increased. Radial extracorporeal shock wave therapy directly interfering with the head of the affected side can reduce the expression of miR-124, increase the number of neural stem cells, and improve impaired nerve function.

Key words: stem cells, neural stem cells, radial extracorporeal shock wave therapy, miR-124, microRNAs, cerebral ischemia, cerebral infarction, middle cerebral artery occlusion, rats

CLC Number:

Li Jie, Ma Yuewen, Kang Nan, Zhang Jing, Zhang Yu. Radial extracorporeal shock wave therapy promotes the proliferation of neural stem cells in hippocampus of cerebral infarction rats and inhibits miR-124 expression[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(31): 4981-4987.

Figures/Tables 6

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