Chinese Journal of Tissue Engineering Research ›› 2019, Vol. 23 ›› Issue (14): 2169-2176.doi: 10.3969/j.issn.2095-4344.1669
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Li Ying1, You Yapeng1, Li Baoe2, Song Yunjia1, Ma Aobo1, Chen Bo1, Han Wen1, Li Changyi1
Received:
2018-12-26
Contact:
Li Changyi, Professor, Doctoral supervisor, Chief physician, Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China
About author:
Li Ying, MD, Associate chief physician, Master’s supervisor, Hospital of Stomatology, Tianjin Medical University, Tianjin 300070, China
Supported by:
the National Natural Science Foundation of China, No. 81870809, 31470920 (to LCY), and 81500886 (to LY); the Natural Science Foundation of Tianjin, No. 16JCYBJC28700 (to LY); the Natural Science Foundation of Hebei Province, No. E2017202032 (to LY)
CLC Number:
Li Ying, You Yapeng, Li Baoe, Song Yunjia, Ma Aobo, Chen Bo, Han Wen, Li Changyi. Type I collagen combined titanium dioxide nanotube composite coating modified titanium surface improves osteoblast adhesion and osseointegration[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(14): 2169-2176.
2.4 各组细胞骨架和黏附斑蛋白染色结果 图4展示的是成骨细胞在各组试样表面的骨架铺展形态和黏附斑蛋白表达情况。由图4A可见,细胞在纯钛表面沿打磨划痕方向伸展,该现象可能是由接触诱导引起;成骨细胞在纳米管表面的铺展明显优于纯钛组,呈现出各个方向伸展的骨架形态,黏附斑蛋白染色较之纯钛组明显;值得注意的是,胶原/纳米管组表面的细胞铺展和延伸更加充分,肌动蛋白纤维呈各向随机排列的特点更加明显,同时黏附斑蛋白染色与纯钛组和纳米管组相比较均明显增强。图4B显示的是细胞总面积和细胞核面积之比(C/N比)的定量分析结果,胶原/纳米管组、纳米管组的C/N比分别是纯钛组的1.7倍和1.4倍。图4C进一步分析了各组试样表面细胞黏附斑染色荧光强度,胶原/纳米管组、纳米管组的黏附斑蛋白染色荧光强度分别为纯钛组强度值的3.5倍和2.3倍。"
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