Chinese Journal of Tissue Engineering Research ›› 2018, Vol. 22 ›› Issue (25): 3937-3943.doi: 10.3969/j.issn.2095-4344.0952
Hou Jing-ying, Long Hui-bao, Chen Xu-xiang, Wang Lei, Guo Tian-zhu, Zheng Shao-xin, Zhong Ting-ting, Wu Quan-hua, Wu Hao, Wang Tong
Revised:
2018-03-22
Online:
2018-09-08
Published:
2018-09-08
Contact:
Hou Jing-ying, Department of Emergency, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China
About author:
Hou Jing-ying, M.D., Attending physician, Department of Emergency, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou 510120, Guangdong Province, China
Supported by:
the National Natural Science Foundation of China, No. 81700242; the Science and Technology Research Project of Guangdong Province, No. 2017A020215176; the Medical Science Research Foundation of Guangdong Province, No. A2016264, A2017001
CLC Number:
Hou Jing-ying, Long Hui-bao, Chen Xu-xiang, Wang Lei, Guo Tian-zhu, Zheng Shao-xin, Zhong Ting-ting, Wu Quan-hua, Wu Hao, Wang Tong. Apelin combined with bone marrow mesenchymal stem cells transplantation improves cardiac function after myocardial infarction[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(25): 3937-3943.
2.1 实验动物数量分析 50只C57BL/6小鼠全部存活,均进入结果分析。 2.2 各组心功能比较 小鼠心肌梗死模型建立后进行心脏超声检测,结果显示各组小鼠左室舒张末期内径、左室收缩末期内径、左室舒张末期室间隔厚度、左室收缩末期室间隔厚度、左室射血分数之间差异均无显著性意义(P > 0.05,表1);在经过相关治疗后2周再次检测心功能,结果显示:与治疗前相比较,MSCs组、MSCs+apelin组、sh-APJ MSCs+apelin组以及apelin组左室舒张末期内径、左室收缩末期内径明显下降,左室射血分数明显增高(P < 0.01,表1和表2),而PBS组治疗前后相比各指标差异无显著性意义(P > 0.05,表1和表2)。与PBS组相比较,其余各组左室舒张末期内径、左室收缩末期内径明显下降,左室射血分数明显增高(P < 0.01,表2);与MSCs组、sh-APJ MSCs+apelin组及apelin组相比较,MSCs+apelin组左室舒张末期内径、左室收缩末期内径明显下降,左室射血分数明显增高(P < 0.01,表2);与MSCs组相比较,apelin组左室舒张末期内径、左室收缩末期内径增加,左室射血分数下降,差异有显著性意义(P < 0.05)。 2.3 MSCs生存和血管再生情况 与MSCs组、sh-APJ MSCs+apelin组相比较,MSCs+apelin组梗死边缘区PKH26(+)以及CD31(+)细胞的平均光密度值显著增高(P < 0.01,图1),PKH26和CD31双阳性细胞占PKH26(+)细胞的比例显著增多(P < 0.01,图1),差异有显著性意义(P < 0.01,图1),而sh-APJ MSCs+apelin组与MSCs组相比较,在上述指标方面差异均无显著性意义(P > 0.05)。 2.4 治疗后2周梗死边缘区APJ、AKT、pAKT以及VEGFA表达 与PBS组相比较,其他组APJ、AKT、pAKT以及VEGFA表达明显增高(P < 0.01,图2);与MSCs组和sh-APJ MSCs+apelin组相比,MSCs+apelin组APJ、AKT、pAKT以及VEGFA的蛋白表达水平明显增加(P < 0.01,图2),而MSCs组、sh-APJ MSCs+apelin组以及apelin组之间上述指标表达差异无显著性意义(P > 0.05,图2)。"
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