Adenovirus transfection of MafA induces human umbilical cord mesenchymal stem cells to express pancreatic cell-specific genes

doi:10.3969/j.issn.2095-4344.2017.09.011

Abstract

Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (HUMSCs) can differentiate into insulin-producing cells after induced by chemical drugs or co-culture methods, but insulin secretion is extremely low. Therefore, to induce mature pancreatic beta cell differentiation from stem cells by adenovirus transfection of specific genes involved in the development of pancreas is a research hotspot in recent years.
OBJECTIVE: To study the differentiation potential of HUMSCs into insulin-producing cells after transfection with musculoaponeurotic fibrosarcoma oncogene homologue A (MafA).
METHODS: Ad-MafA-EGFP was transferred into passage 3 HUMSCs. After 7 days of induction, changes of cell morphology were observed by inverted phase contrast microscope. Expression of pancreatic cell-specific genes (glucagon, PDX1, Nkx2.2) was detected by PCR technique.
RESULTS AND CONCLUSION: After Ad-MafA-EGFP transfection, no significant morphological changes were observed in the HUMSCs under inverted phase contrast microscope. It was confirmed by fluorescence microscope that Ad-MafA-EGFP was transferred into the HUMSCs. After induction, the expression of human pancreatic precursor cell-related genes, including glucagon, PDX1 and Nkx2.2, was increased as detected by PCR. To conclude, these findings could provide experimental evidence for further differentiation and maturation of pancreatic β cells from HUMSCs.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cells, Maf Transcription Factors, Insulin-Secreting Cells, Tissue Engineering

CLC Number:

R394.2

Wang Hong-wu, Ni Ping, Lai Xiu-lan, Feng Xue-yong, Lin Li-min, Ma Lian. Adenovirus transfection of MafA induces human umbilical cord mesenchymal stem cells to express pancreatic cell-specific genes[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(9): 1368-1372.

Figures/Tables 1

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