Purification and cytokine profile of autologous conditioned serum

doi:10.3969/j.issn.2095-4344.2017.24.021

Abstract

Abstract:

BACKGROUND: Autologous conditioned serum (ACS) is a new and safe biological therapy method mainly used for osteoarthritis, rheumatoid arthritis and other similar diseases.
OBJECTIVE: To explore the purification method and cytokine profile of ACS.
METHODS: Blood obtained equipment (containing 3 mm of glass beads) was used to collect peripheral blood samples that were mixed uniformly and then placed in a 37 ℃ thermostatic incubator for 24 hours, finally filtered to obtain ACS. The quantitative determination of interleukin (IL)-1Ra, IL-6, IL-8, and IL-1β was conducted, and the 36 kinds of cytokines were semi-quantitatively detected by cytokine antibody array.
RESULTS AND CONCLUSION: The levels of various cytokines in ACS were obviously increased, among which, IL-1Ra level (important for alleviating osteoarthritis) was increased from 140.25 ng/L to 1 125 ng/L(increased by above 8 times); IL-6 level was increased from 389.5 ng/L to 2 802 ng/L; IL-8 level was not detected firstly and finally increased to 2 822 ng/L. Cytokine antibody array results showed that at least 10 kinds of cytokines in ACS were changed, and most of cytokines were on a rise, and few in decline. These results indicate that the ACS contains IL-1Ra and a variety of cytokines, and some chemokines in the ACS recruit more immune cells to the inflammation sites and accelerate the inflammatory process, further exerting therapeutic effects.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Serum, Cytokines, Osteoarthritis, Tissue Engineering

CLC Number:

R318

Chen Xi, Wang Jian, Li Yi-jia. Purification and cytokine profile of autologous conditioned serum[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(24): 3894-3899.

Figures/Tables 1

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