Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (2): 182-186.doi: 10.3969/j.issn.2095-4344.2017.02.004

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Bone biomaterial composited with human bone marrow mesenchymal stem cells for large costal defects

Wang Jun-gang1, Li Cong-cong2, Mao Guang-xian3, Zhang Jie1, Yang Cui1
  

  1. 1Department of Thoracic Surgery, 2First Special Ward, Affiliated Hospital of Zhengzhou University & Nanyang City Central Hospital, Nanyang 473000, Henan Province, China; 3Department of Thoracic Surgery, Peking University Shenzhen Hospital, Shenzhen 518036, Guangdong Province, China
  • Received:2016-10-26 Online:2017-01-18 Published:2017-02-27
  • About author:Wang Jun-gang, Attending physician, Department of Thoracic Surgery, Affiliated Hospital of Zhengzhou University & Nanyang City Central Hospital, Nanyang 473000, Henan Province, China
  • Supported by:

    the Science and Technology Program of Shenzhen, No. JCYJ20140415162338820; the Project of Medical and Health Research of Shenzhen, No. 201302068

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Abstract:

BACKGROUND: Tissue-engineered biomaterials have the similar structure and function with autologous tissues.
OBJECTIVE: To explore the osteoinduction of the bone biomaterial composited with rat bone marrow mesenchymal stem cells in the treatment of large costal defects.
METHODS: Forty Wistar rats were enrolled used for the preparation of right large costal defect models, and then randomized into two groups, followed by the implantation of calcium chloride-sodium alginate gel (control group) or chloride-sodium alginate-bone marrow mesenchymal stem cells (experimental group). At 2, 4 and 8 weeks after implantation, chest X-ray radiograph and histological examination of the defect region were conducted.
RESULTS AND CONCLUSION: X-ray showed that in the experimental group, the defect area had no significant changes at the 2nd week after implantation until the formation of few bones at the 4th week; and at the 8th week, both ends of the defect region gradually connected, and newly formed bones were full of the defect. In contrast, the defect region in the control group showed no obvious bone healing, and both ends of the defect closed and osteosclerosis occurred. In the experimental group, there were a small amount of fibrous tissues and numerous inflammatory cells infiltratied in the material compartment, and no connection occured between the material and broken ends; there were numerous inflammatory cells but no bone tissues in the control group at the 2nd week. At the 4th week, the scaffold degraded gradually and abundant bone tissues were seen in the experimental group; the scaffold degraded little, and bone tissues aggregatied at the both defect ends in the control group. Up to the 8th week, the two kinds of scaffolds degraded mostly. A large number of bone tissues and trabeculae formed and the both defect ends were connected with the newly formed bones in the experimental groups, while in the control group, osteosclerosis appeared at both ends of the defect. To conclude, the bone biomaterial composited with rat bone marrow mesenchymal stem cells promotes the repair of large costal defects.

Key words: Alginates, Mesenchymal Stem Cells, Tissue Engineering

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