Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (2): 187-191.doi: 10.3969/j.issn.2095-4344.2017.02.005

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In vitro toxic effect of polymethylmethacrylate bone cement on lung cancer cells

Pan Yuan-xing, Mi Chuan, Shi Xue-dong, Wang Bing, Cui Yun-peng, Lin Yun-fei
  

  1. Department of Orthopaedics, Peking University First Hospital, Beijing 100083, China
  • Received:2016-10-21 Online:2017-01-18 Published:2017-02-27
  • Contact: Mi Chuan, Chief physician, Associate professor, Department of Orthopaedics, Peking University First Hospital, Beijing 02110003, China
  • About author:Pan Yuan-xing, M.D., Department of Orthopaedics, Peking University First Hospital, Beijing 02110003, China

Abstract:

BACKGROUND: Percutaneous vertebroplasty is a minimally invasive treatment for spinal metastasis tumor, but the mechanism of the injected polymethylmethacrylate (PMMA) bone cement in the treatment of tumor is not fully understood.
OBJECTIVE: To explore the cytotoxicity of PMMA bone cement and its monomers on tumor cells.
METHODS: PMMA extracts in the wire drawing and curing periods and different mass concentrations of monomer dilutions were co-cultured with human lung cancer cells spc-A1. The cell morphology was observed using inverted microscope, the absorbance (A) values were detected by cell counting kit- 8 assay, the relative growth rate was calculated, and the toxicity of PMAA bone cement and its monomers was evaluated at 1 and 3 days of culture, respectively.
RESULTS AND CONCLUSION: At 1 day of culture, the absorbance values in the 1 and 10 g/L groups were significantly lower than that in the negative control group (P < 0.01). The absorbance values in the 1 g/L, 10 g/L, 100 mg/L and wire drawing extract groups were significantly lower than those in the negative control group at 3 days of culture (P < 0.05). The relative growth rate in the 1 and 10 g/L groups was 26%-29%, and the level of toxicity was grade 4 after 1-day culture; at 3 days, the relative growth rate was decreased to 12%-16%, and the level of toxicity was grades 4-5. After 3-day culture, the level of toxicity reached to grade 2 in the 100 mg/L and wire drawing extract groups. These results indicate that PMMA bone cement in wiredrawing period and its monomers exert toxic effects on tumor cells.

Key words: Polymethacrylic Acids, Vertebroplasty, Neoplasms, Tissue Engineering

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