In vitro degradation of concentrated growth factor fibrin versus platelet-rich fibrin

doi:10.3969/j.issn.2095-4344.2017.14.017

Abstract

Abstract:

BACKGROUND: The degradation speed of biological materials is critical for the clinical use of guided bone regeneration. The partial biological characteristics and treatment efficacy of concentrate growth factor (CGF) fibrin have been explored preliminarily, but its degradation properties have not yet been reported.
OBJECTIVE: To explore the degradation properties of CGF fibrin and platelet-rich fibrin (PRF) in artificial saliva and compare the degradation speed of these two biological products.
METHODS: Ten volunteers were selected, and 18 mL of venous blood from each volunteer was extracted and stored in two vacuum blood collectors. The blood samples were then placed into the drum of the Medifuge centrifugal acceleration machine, to separate CGF fibrin and PRF specimens following the preparation process, respectively. Both CGF fibrin and PRF specimens were respectively made into bulk and membranoid, and were then immersed in artificial saliva under 37 ℃. The mass of the bulk specimens and area of the membranoid specimens were measured regularly, and the degradation processes of CGF and PRF were recorded. The degradation curves were drawn to compare the degradation speed of CGF fibrin and PRF.
RESULTS AND CONCLUSION: The mass of CGF fibrin and PRF showed no significant difference at the 5th day (P > 0.05), while the mass of CGF fibrin was higher than that of PRF at the 3rd, 4th and 6th days (P < 0.05). The residual area of CRF was significantly larger than that of PRF at posttreatment 1-6 days (P < 0.05). To conclude, the degradation speed of bulk or membranoid CRF is slow than that of PRF in artificial saliva. The higher the fibrin content is, the slower the degradation ability is, indicating the strong bioreproductive function.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Saliva, Artificial, Guided Tissue Regeneration, Blood Palates, Fibrin, Tissue Engineering

CLC Number:

R318

Li Yong-bin, Sun Ying-chun, Wei Rong-zhi, Yang Jian, Sheng Hai-ying, Chen Jing. In vitro degradation of concentrated growth factor fibrin versus platelet-rich fibrin[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(14): 2234-2240.

Figures/Tables 4

组内进一步方差分析比较结果显示：浓缩生长因子纤维蛋白质量在实验前7 d，除第3天与第2天相比表现为平缓下降，其余时间点均表现为显著下降(P < 0.05)；从第8天开始，浓缩生长因子纤维蛋白质量残余量很少，同时维持不变(P > 0.05)；第10天浓缩生长因子纤维蛋白完全降解。富血小板纤维蛋白质量在实验前6 d随实验周期的推移表现显著下降(P < 0.05)，第7天时保持与第6天持平，第8天降解完全。组间进一步t 检验比较结果显示：组1与组3中标本块的初始质量差异无显著性意义(P > 0.05)；实验开始后，浓缩生长因子纤维蛋白与富血小板纤维蛋白在实验第5天标本块的质量差异无显著性意义(P > 0.05)，其余时间点两者质量均表现为差异有显著性意义(P < 0.05)。实验标本种类与实验周期之间存在交互作用(F=36.071，P <0.05)。 2.2 浓缩生长因子纤维蛋白和富血小板纤维蛋白膜状标本面积比较见表3及图3。膜状标本重复测量方差分析组内比较结果显示：随着时间的变化，组2与组4中膜状标本的面积都发生了明显的变化(F=9 750.945，P < 0.05)。组间比较结果显示，浓缩生长因子纤维蛋白与富血小板纤维蛋白膜状标本在相同时间点剩余面积差异有显著性意义(F=3 369.460， P < 0.05)。组内进一步方差分析比较结果显示：浓缩生长因子纤维蛋白膜状标本面积在实验前6 d表现为显著下降(P < 0.05)；实验第7天，浓缩生长因子纤维蛋白完全降解。富血小板纤维蛋白膜状标本面积在实验前4 d随实验周期的推移表现为显著下降(P < 0.05)，第5天时降解完全。组间进一步t 检验比较结果显示：组2与组4中膜状标本的初始面积差异无显著性意义(P > 0.05)；实验开始后，浓缩生长因子纤维蛋白与富血小板纤维蛋白膜状标本剩余面积表现为差异有显著性意义(P < 0.05)。实验膜状标本种类与实验周期之间存在交互作用(F=446.457，P < 0.05)。 "

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