Large-diameter TiO2 nanotubes with nano-hydroxyapatite offer an improvement in bone-forming ability

doi:10.3969/j.issn.2095-4344.2017.14.009

Abstract

Abstract:

BACKGROUND: Both hydroxyapatite (HA) and large diameter TiO₂ nanotubes have excellent biocompatibility, but bone-forming ability of nano-HA (nHA) deposited large diameter TiO₂ nanotubes is rarely reported.
OBJECTIVE: To evaluate the bone-forming ability of nHA/large-diameter TiO₂ nanotube composite coating.
METHODS: Large-diameter TiO₂ nanotubes were prepared by anodic oxidation method, and then nHA was electrochemically deposited on the surface of TiO₂ nanotubes. Preosteoblasts MC3T3-E1 were co-cultured with the nHA/large diameter TiO₂ nanotube composite, pure titanium and TiO₂ nanotube coatings, respectively. At 0.5, 1, 2 hours after culture, the initial cell adhesion was observed. At 1, 3, 5 day after culture, cell proliferation was assessed. At 2 days after culture, cell morphology was observed. At 3 and 7 days after osteogenic induction, intracellular alkaline phosphatase activity was detected. At 14 days after osteogenic induction, mineralization of extracellular matrix was detected.
RESULTS AND CONCLUSION: (1) After 2 hours of culture, the number of adherent cells on the composite coating was significantly lower than that on the TiO₂ nanotube coating (P < 0.05), but slightly higher than that on the pure titanium coating with no statistical difference. (2) After 1, 3, 5 days of culture, the cell proliferation on the composite coating was significantly lower than that on the TiO₂ nanotube coating (P < 0.05), but slightly higher than that on the pure titanium with no statistical difference. (3) The cells on the pure titanium showed a spindle-shape, while those on the TiO₂ nanotube coating processed filopodia. The cells on the composite coating showed polygonal shape with a larger number of filopodia. (4) The intracellular alkaline phosphatase activity of the composite coating group was significantly higher than that of the pure titanium group and TiO₂ nanotube group. The trend of mineralization of extracellular matrix was ranked from high to low: the composite coating group > TiO₂ nanotube group > pure titanium group. To conclude, the nHA/large diameter TiO₂ nanotube composite coating not only has good biocompatibility, but also has the ideal ability to promote bone formation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Durapatite, Nanotubes, Osteogenesis, Tissue Engineering

CLC Number:

R318

Lei Ze-ming, Zhang Hang-zhou, Tian Ang, You Jun-hua, Shi Xiao-guo, Liu Xing-wang, Wei Bo, Bai Xi-zhuang. Large-diameter TiO₂ nanotubes with nano-hydroxyapatite offer an improvement in bone-forming ability[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(14): 2186-2191.

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References

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Large-diameter TiO₂ nanotubes with nano-hydroxyapatite offer an improvement in bone-forming ability

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