Local transplantation of human umbilical cord mesenchymal stem cells at different time after spinal cord injury in rats: a histological observation

doi:10.3969/j.issn.2095-4344.2016.45.004

Abstract

Abstract:

BACKGROUND: Spinal cord injury (SCI) is a disease causing a variety of motor and sensory dysfunctions, abnormal muscle tone and pathological reflex. Clinically, human umbilical cord mesenchymal stem cell transplantation has become an employed therapy for SCI.
OBJECTIVE: To investigate the effect of local transplantation of human umbilical cord mesenchymal stem cells in different time after spinal cord injury in rats.
METHODS: 100 SPF male adult Sprague-Dawley rats were randomly divided into five groups: blank control group, SCI group, post-SCI 3-, 7-, 21-day transplantation groups (n=20 per group). Animal models of T₁₀ SCI were made by Allen’s method in the latter four groups, and rats in the three transplantation groups were given HUCMSCs transplantation at 3, 7, 21 days after SCI, respectively.
RESULTS AND CONCLUSION: Compared with the SCI group, improved motor function scores, decreased interleukin-2 level, and increased serum interleukin-10 level were observed in the three transplantation groups at 49 days after modeling, indicating SCI was improved significantly in the three transplantation groups, especially in the post-SCI 7-day transplantation group. These findings suggest that human umbilical cord mesenchymal stem cell transplantation for SCI repair improves the movement function of rats, and cell transplantation at 7 days after modeling has achieved best outcomes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Interleukins, Spinal Cord Injuries, Tissue Engineering

CLC Number:

R394.2

Chen Sheng, Jin Zheng-shuai. Local transplantation of human umbilical cord mesenchymal stem cells at different time after spinal cord injury in rats: a histological observation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(45): 6714-6719.

Figures/Tables 2

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