Moderate cyclic compressive stress accelerates anabolism of articular chondrocytes by affecting cytoskeleton

doi:10.3969/j.issn.2095-4344.2016.37.006

Abstract

Abstract:

BACKGROUND: Different mechanical stimulations may have an effect on the level of metabolism of chondrocytes, but the effect is not clear.
OBJECTIVE: To investigate expression level changes in metabolic genes that participate in cartilage cell decomposition and synthesis under compressive stress and tensile stress conditions.
METHODS: We obtained articular chondrocytes from 2-week-old Sprague-Dawley rats. Primary cultured chondrocytes were identified. Passage one chondrocytes received cyclic tensile stress and cyclic compressive stress of 3% and 7%, respectively, so as to measure articular changes in chondrocytes-related genes.
RESULTS AND CONCLUSION: When chondrocytes were subjected to cyclic tensile stress of 3%, synthetic metabolic gene collagen types I and II and proteoglycan mRNA expression levels were decreased. If 3% cyclic compressive stress was applied, proteoglycan mRNA expression levels were increased, and type I collagen mRNA expression levels were decreased (P < 0.001), and matrix metalloproteinase-13 mRNA expression levels were reduced (P < 0.01). When strain reached 7%, cyclic tensile stress and compressive stress could lead to a general decrease in anabolism-related genes. The former could also make matrix metalloproteinase-13 mRNA expression levels increased (P < 0.05). 3% cyclic compression ratio and 3% cyclic stretch made cytoskeleton become oval. These results indicated that in vitro, proper cyclic compressive stress is beneficial to maintain the growth characteristics of articular chondrocytes in rats. Small tensile stress can decrease the synthesis ability of chondrocytes. The effect of stress may be caused by changing the cytoskeleton.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Chondrocytes, Mechanics, Metabolism, Tissue Engineering

CLC Number:

R318

Mo Jun, Chen Ying, Zhong Dong-yan, Yang Hui-lin, Luo Zong-ping. Moderate cyclic compressive stress accelerates anabolism of articular chondrocytes by affecting cytoskeleton[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.37.006.

Figures/Tables 1

References

[1] Visser AW, de Mutsert R, le Cessie S, et al. The relative contribution of mechanical stress and systemic processes in different types of osteoarthritis: the NEO study. Ann Rheum Dis. 2015;74(10):1842-1847.
[2] Anderson JJ, Felson DT. Factors associated with osteoarthritis of the knee in the first national Health and Nutrition Examination Survey (HANES I). Evidence for an association with overweight, race, and physical demands of work. Am J Epidemiol. 1988;128(1):179-189.
[3] Jungmann PM, Kraus MS, Alizai H, et al. Association of metabolic risk factors with cartilage degradation assessed by T2 relaxation time at the knee: data from the osteoarthritis initiative. Arthritis Care Res (Hoboken). 2013;65(12):1942-1950.
[4] Lee SH, Lee JH, Ahn SE, et al. Correlation between Quadriceps Endurance and Adduction Moment in Medial Knee Osteoarthritis. PLoS One. 2015;10(11): e0141972.
[5] O'Connell M, Farrokhi S, Fitzgerald GK. The role of knee joint moments and knee impairments on self-reported knee pain during gait in patients with knee osteoarthritis. Clin Biomech (Bristol, Avon). 2016;31:40-46.
[6] Wong M, Siegrist M, Goodwin K. Cyclic tensile strain and cyclic hydrostatic pressure differentially regulate expression of hypertrophic markers in primary chondrocytes. Bone. 2003;33(4):685-693.
[7] Bleuel J, Zaucke F, Brüggemann GP, et al. Moderate cyclic tensile strain alters the assembly of cartilage extracellular matrix proteins in vitro. J Biomech Eng. 2015;137(6):061009.
[8] Mabvuure N, Hindocha S, Khan WS. The role of bioreactors in cartilage tissue engineering. Curr Stem Cell Res Ther. 2012;7(4):287-292.
[9] Jakob M, Démarteau O, Schäfer D, et al. Enzymatic digestion of adult human articular cartilage yields a small fraction of the total available cells. Connect Tissue Res. 2003;44(3-4):173-180.
[10] Duan W, Wei L, Cao X, et al. Effect of the disruption of three cytoskeleton components on chondrocyte metabolism in rabbit knee cartilage. Chin Med J (Engl). 2014;127(21):3764-3770.
[11] Duan W, Wei L, Zhang J, et al. Alteration of viscoelastic properties is associated with a change in cytoskeleton components of ageing chondrocytes from rabbit knee articular cartilage. Mol Cell Biomech. 2011; 8(4):253-274.
[12] Madhavan S, Anghelina M, Rath-Deschner B, et al. Biomechanical signals exert sustained attenuation of proinflammatory gene induction in articular chondrocytes. Osteoarthritis Cartilage. 2006;14(10):1023-1032.
[13] Luo Y, Zhang L, Wang WY, et al. Alendronate retards the progression of lumbar intervertebral disc degeneration in ovariectomized rats. Bone. 2013;55(2): 439-448.
[14] Nam J, Rath B, Knobloch TJ, et al. Novel electrospun scaffolds for the molecular analysis of chondrocytes under dynamic compression. Tissue Eng Part A. 2009; 15(3):513-523.
[15] Amini S, Mortazavi F, Sun J, et al. Stress relaxation of swine growth plate in semi-confined compression: depth dependent tissue deformational behavior versus extracellular matrix composition and collagen fiber organization. Biomech Model Mechanobiol. 2013; 12(1):67-78.
[16] Amini S, Veilleux D, Villemure I. Tissue and cellular morphological changes in growth plate explants under compression. J Biomech. 2010;43(13):2582-2588.
[17] Wu L, Gonzalez S, Shah S, et al. Extracellular matrix domain formation as an indicator of chondrocyte dedifferentiation and hypertrophy. Tissue Eng Part C Methods. 2014;20(2):160-168.
[18] Demoor M, Ollitrault D, Gomez-Leduc T, e al. Cartilage tissue engineering: molecular control of chondrocyte differentiation for proper cartilage matrix reconstruction. Biochim Biophys Acta. 2014;1840(8): 2414-2440.
[19] Gründer T, Gaissmaier C, Fritz J, et al. Bone morphogenetic protein (BMP)-2 enhances the expression of type II collagen and aggrecan in chondrocytes embedded in alginate beads. Osteoarthritis Cartilage. 2004;12(7):559-567.
[20] Jonitz A, Lochner K, Tischer T, et al. TGF-β1 and IGF-1 influence the re-differentiation capacity of human chondrocytes in 3D pellet cultures in relation to different oxygen concentrations. Int J Mol Med. 2012; 30(3):666-672.
[21] Pei M, Seidel J, Vunjak-Novakovic G, et al. Growth factors for sequential cellular de- and re-differentiation in tissue engineering. Biochem Biophys Res Commun. 2002;294(1):149-154.
[22] Loeser RF. Integrins and chondrocyte-matrix interactions in articular cartilage. Matrix Biol. 2014;39: 11-16.
[23] Mao Y, Schwarzbauer JE. Fibronectin fibrillogenesis, a cell-mediated matrix assembly process. Matrix Biol. 2005;24(6):389-399.
[24] Maenz S, Hennig M, Mühlstädt M, et al. Effects of oxygen plasma treatment on interfacial shear strength and post-peak residual strength of a PLGA fiber-reinforced brushite cement. J Mech Behav Biomed Mater. 2016;57:347-358.
[25] Gao Y, Liu S, Huang J, et al. The ECM-cell interaction of cartilage extracellular matrix on chondrocytes. Biomed Res Int. 2014;2014:648459.
[26] Chen C, Wei X, Lv Z, et al. Cyclic Equibiaxial Tensile Strain Alters Gene Expression of Chondrocytes via Histone Deacetylase 4 Shuttling. PLoS One. 2016; 11(5): e0154951.
[27] 余晓明,孟昊业,孙振,等.生物反应器中的力学刺激促进组织工程软骨再生[J].中国组织工程研究,2016,20(2): 185-190.
[28] Matsukawa M, Fukuda K, Yamasaki K, et al. Enhancement of nitric oxide and proteoglycan synthesis due to cyclic tensile strain loaded on chondrocytes attached to fibronectin. Inflamm Res. 2004;53(6):239-244.
[29] Shimizu A, Watanabe S, Iimoto S, et al. Interleukin-4 protects matrix synthesis in chondrocytes under excessive mechanical stress in vitro. Mod Rheumatol. 2004;14(4):296-300.
[30] Bleuel J, Zaucke F, Brüggemann GP, et al. Effects of cyclic tensile strain on chondrocyte metabolism: a systematic review. PLoS One. 2015;10(3):e0119816.
[31] Kaviani R, Londono I, Parent S, et al. Compressive mechanical modulation alters the viability of growth plate chondrocytes in vitro. J Orthop Res. 2015; 33(11):1587-1593.
[32] Kaviani R, Londono I, Parent S, et al. Growth plate cartilage shows different strain patterns in response to static versus dynamic mechanical modulation. Biomech Model Mechanobiol. 2015. in press.
[33] Sergerie K, Parent S, Beauchemin PF, et al. Growth plate explants respond differently to in vitro static and dynamic loadings. J Orthop Res. 2011;29(4): 473-480.