Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro

doi:10.3969/j.issn.2095-4344.2016.37.002

Abstract

Abstract:

BACKGROUND: It has been proved that Rhizoma Chuanxiong can promote osteoblast proliferation and differentiation.
OBJECTIVE: To further observe the effects of tetramethylpyrazine (Ligustrazine) extracted from Rhizoma Chuanxiong on osteoblast proliferation and type I collagen expression.
METHODS: Mouse osteoblast cell line MC3T3-E1 was cultured in medium containing 1, 5, 10, 15 and 20 mg/L Ligustrazine, respectively. Osteoblast proliferation was detected by cell counting kit-8 assay, and alkaline phosphatase activity and type I collagen level measured using ELISA method.
RESULTS AND CONCLUSION: Different concentrations of Ligustrazine all significantly promoted the osteoblast proliferation and type I collagen level compared with the blank control group (P < 0.005). The activity of alkaline phosphatase in all Ligustrazine groups except 15 and 20 mg/L groups was significantly higher than that in the blank control group (P < 0.005). These results show that Ligustrazine isolated from Rhizoma Chuanxiong can effectively promote the osteoblast proliferation and type I collagen expression, with the optimum concentration of 10 mg/L.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoblasts, Cell Proliferation, Alkaline Phosphatase, Collagen Type I, Drugs, Chinese Herbal, Tissue Engineering

CLC Number:

R318

Lu Wei, Guo Rui, Zhao Gang. Effects and optimum concentration of Rhizoma Chuanxiong on osteoblasts in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(37): 5483-5488.

Figures/Tables 3

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