Effects of acupuncture and rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin at the injury site of cerebral palsy rats

doi:10.3969/j.issn.2095-4344.2016.27.007

Abstract

Abstract:

BACKGROUND: Acupuncture + rehabilitation therapy is the most effective treatment for cerebral palsy. Growth associated protein-43 and synaptophysin were characteristic markers for neurodevelopment and synaptic density, and involved in nerve cell growth, repair, regeneration and synapse remodeling.
OBJECTIVE: To study the effects of acupuncture + rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin in cerebral palsy rats and to investigate the mechanism of acupuncture + rehabilitation therapy on cerebral palsy.
METHODS: The 125 rats were randomly divided into five groups: sham group, model group, rehabilitation group, acupuncture group, and acupuncture + rehabilitation group. Rat models of cerebral palsy were established in model group, rehabilitation group, acupuncture group, and acupuncture + rehabilitation group. Rat models in the sham and model groups did not receive treatment. Rat models in the rehabilitation group underwent rehabilitation training. Rat models in the acupuncture group were subjected to acupuncture. Rat models in the acupuncture + rehabilitation group underwent rehabilitation training and acupuncture.
RESULTS AND CONCLUSION: (1) Motor function: The motor function scores were better in the acupuncture + rehabilitation group than in the model, acupuncture, and rehabilitation groups (P < 0.05). (2) Growth associated protein-43 and synaptophysin expression: immunohistochemical staining revealed that compared with the model group, growth associated protein-43 expression at 2-4 weeks and synaptophysin expression at 4 and 5 weeks were higher in the acupuncture and acupuncture + rehabilitation groups (P < 0.05); the increase in above expression was most significant in the acupuncture + rehabilitation group (P < 0.05). (3) Results confirmed that acupuncture combined with rehabilitation promoted growth associated protein-43 and synaptophysin expression in rats with cerebral palsy and exerted neuroprotective effect.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Growth Associated Protein-43, Models, Animal

CLC Number:

R318

Hou Yu-jin, Kang Hao-peng. Effects of acupuncture and rehabilitation therapy on the expression of growth associated protein-43 and synaptophysin at the injury site of cerebral palsy rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(27): 3999-4005.

Figures/Tables 2

References

[1] Holtzman DM, Sheld RA, Jaffe W, et al. Nerve growth factor protects the neonatal brain again hypoxic- ischemic injury. Ann Neurol. 1996;39:114.
[2] 华兴邦,李辞蓉,周浩良,等.大鼠穴位图谱的研制[J].实验动物与动物实验,1991,1:1.
[3] 吴云.小儿脑性瘫痪的发病机制及诊治进展[J].安徽医学, 2011,32(6):859-862.
[4] 怀智勇.小儿痉挛型脑性瘫痪的中西医治疗概况[J].按摩与康复医学.2012.03(1):47-48.
[5] 唐秀梅,钟陶.感觉统合训练联合音乐疗法对小儿脑性瘫痪功能康复的效果观察[J].现代诊断与治疗, 2013, 24(10):2385-2389.
[6] 侯晓晖,李裕和,王春阳,等.Halliwick技术对学龄期脑性瘫痪儿童平衡和粗大运动功能的影响[J].中国运动医学杂志, 2013,32(10):875-880.
[7] 刘启雄,万子超.核心稳定性训练对脑性瘫痪患儿粗大运动的影响[J].鄂州大学学报,2013,20(5):70-71.
[8] 魏明香,林小芹,黄翠梅,等.引导式教育对脑性瘫痪儿童智能发育的影响分析[J].临床医学工程,2013,20(4): 434-435.
[9] 汤健,张跃,朱敏,等.头针对脑性瘫痪儿童认知功能和脑干听觉诱发电位的影响[J].南京中医药大学学报,2013, 29(6):532-534.
[10] 谭晓如,赵伊黎,谢洁珊,等.针刺配合功能训练改善小儿脑性瘫痪精细运动功能临床观察[J].中医儿科杂志,2013, 9(5):40-43.
[11] 何金华,张惠佳,王益梅,等.针刺配合强制性诱导运动疗法对偏瘫型脑性瘫痪患儿上肢功能的影响[J].中国康复医学杂志,2013,28(9):864-866.
[12] 刘辉,徐琳,董宝强.穴位埋线治疗不随意运动型脑性瘫痪随机平行对照研究[J].实用中医内科杂志,2013,27(6): 113-115.
[13] 黄启军,杨杰,张燕君,等.华佗磁疗贴压耳穴对200例脑性瘫痪儿童体质调理的临床疗效观察[J].中医儿科杂志, 2013,9(1):62-64.
[14] 陈玲.补中益气汤内服与药浴对脑性瘫痪膝反张的观察[J].军事医学,2012,36(2):160-161.
[15] 陆春玲,周顺林,郭刚.参鹿启智汤治疗痉挛型脑性瘫痪40例临床观察[J].河北中医,2012,34(1):21-23.
[16] 郑宏,张建奎,雷爽,等.辨证施术推拿按摩对痉挛型脑性瘫痪粗大运动功能及中医证候积分的影响[J].中国康复医学杂志,2013,28(10):952-954.
[17] 刘朝辉,吴夏勃.点穴疗法治疗产伤性痉挛型脑性瘫痪之关节功能障碍的临床观察[J].中国优生优育,2013,19(3): 176-178.
[18] 王雪花.NMES结合运动疗法对脑性瘫痪患儿运动功能的影响[J].中外医学研究,2013,11(33):165.
[19] 徐晨.经颅超低频磁刺激治疗脑性瘫痪患儿的疗效分析[J].中国医药指南,2013,11(23):213.
[20] 刘振寰,张丽红,赵勇.五行体感音乐对痉挛型脑性瘫痪患儿肌张力的影响[J].中国康复理论与实践,2013,19(8): 771-774.
[21] 张丽丽,潘勇,赵斌.康复运动疗法配合针灸治疗脑性瘫痪流涎的疗效观察[J].中国伤残医学,2013,21(5):25-26.
[22] 宋西晓,席慧萍.熏蒸方配合上田正疗法治疗小儿痉挛型脑性瘫痪88例[J].陕西中医,2012,33(7):799-780.
[23] 张红运,杜菲.针刺结合坐位训练治疗脑性瘫痪患儿坐位功能异常的临床研究[J].针刺研究,2013,38(5):403-410.
[24] 唐强,朱琳,王艳,等.穴位埋线治疗不随意运动型脑性瘫痪随机平行对照研究[J].辽宁中医杂志,2013,40(6): 1063-1064.
[25] 熊裕娟,覃蓉,王跑球,等.中药蒸汽浴配合功能训练治疗痉挛型脑性瘫痪59例临床观察[J].中医药导报,2013,19(1): 36-38.
[26] 苏炜,王建文.推拿手法结合康复训练治疗68例痉挛型脑性瘫痪疗效观察[J].甘肃医药,2013,32(11):838-840.
[27] 李爱霞,左月仙,贾革红,等.中医手法介入对脑性瘫痪儿童吞咽障碍康复的疗效观察[J].中国康复理论与实践, 2013, 19(4):375-377.
[28] 李威,章荣,罗亚玲,等.步态诱发功能性电刺激改善痉挛型双瘫型脑性瘫痪患儿下肢运动功能的疗效观察[J].中国康复医学杂志,2013,28(12):1126-1130.
[29] 徐晨.小脑电刺激对脑性瘫痪儿童康复作用的疗效评价[J].中国现代药物应用,2013,7(20):48-49.
[30] 王国洪,姜帅,林瑜.针灸按摩联合溶栓胶囊治疗小儿脑性瘫痪的疗效观察[J].中华中医药学刊,2013,31(5):1191.
[31] 刘云.头穴丛剌结合康复训练对急性脑梗死大鼠神经可塑性影响的研究[D].哈尔滨:黑龙江中医药大学,2004.
[32] 宋畅.头穴丛刺结合康复技术对急性脑梗死患者临床疗效及IL-6、TGF-P1含量影响的研究[D].哈尔滨:黑龙江中医药大学,2008.
[33] 唐强,白晶,王艳,等.头穴丛刺法调控大鼠脑梗死后内源性神经干细胞增殖迁移分化的实验研究[J].中国康复医学杂志,2009,24(8):676-679.
[34] 关莹,张立,邢艳丽,等.针康法对脑卒中后痉挛状态的影响[J].中国康复理论与实践,2011,17(4):325-327.
[35] 刘波,唐强,孔妍.头穴丛刺结合康复训练治疗脑梗死患者运动功能障碍的临床观察[J].针灸临床杂志,2009,25(10): 32-34.
[36] 张慧敏,唐强.针康法治疗脑卒中运动性失语患者79例临床观察[J].中医杂志,2011,52(10):855-858.
[37] 邢艳丽,杨龙,张立,等.头穴丛剌结合康复治疗对脑梗死患者平衡功能影响的研究[J].中国中医药咨讯,2010,2(30): 126-127.
[38] 王艳,张立,李淑荣,等.头穴丛剌结合认知训练对脑梗死患者认知功能障碍的影响[J].中国康复理论与实践,2011,17 (4):316-318.
[39] Mcllvain VA, Robertson DR, Maimone MM, et al. Abnormal thalamocortical pathfinding and terminal arbors lead to enlarged barrels in neonatal GAP-43 heterozygous mice. J Comp Neurol. 2003;462(2): 252-264.
[40] Chen B, Wang JF, Sun X. Regulation of GAP-43 expression by chronic desipramine treatment in rat cultured hippocampal cells. Biol Psychiatry. 2003; 53(6):530-537.
[41] Levy HI, Hanscom B, boden SD. Three-question depression screener used for lumbar disc herniation and spinal stenosis. Spine. 2002;27(11):1232-1237.
[42] Thiel G, Synapsin I. Synapsin II and Synaptophysin: marker proteins of synaptic vesicles. Brain Pathol. 1993;3(1):87-95.
[43] 槐雅萍,杨永轩,贾子善,等.探索学习对局灶性脑梗死大鼠梗死灶周围皮质巢蛋白及突触素表达的影响[J].中国全科医学,2010,13(5):501-505.
[44] Rumajogee P, Bregman T, Miller SP, et al. Rodent Hypoxia-Ischemia Models for Cerebral Palsy Research: A Systematic Review. Front Neurol. 2016.
[45] Kang B, Kim DH, Hong YJ, et al. Complete occlusion of the right middle cerebral artery associated with Mycoplasma pneumoniae pneumonia. Korean J Pediatr. 2016;59(3):149-152.
[46] Placha K, Luptakova D, Baciak L, et al. Neonatal brain injury as a consequence of insufficient cerebral oxygenation. Neuro Endocrinol Lett. 2016;37(2):79-96.
[47] Balakrishnan N, Ericson M, Maggi R, et al. Vasculitis, cerebral infarction and persistent Bartonella henselae infection in a child. Parasit Vectors. 2016;9(1):254.
[48] Wang JH, Zhao M, Bao YC, et al. Effect of Scalp-acupuncture Treatment on Levels of Serum High-sensitivity C-reactive Protein, and Pro-inflammatory Cytokines in Patients with Acute Cerebral Infarction. Zhen Ci Yan Jiu. 2016;41(1):80-84.
[49] Zhang CH, Bian JL, Meng ZH, et al. Tongguan Liqiao acupuncture therapy improves dysphagia after brainstem stroke. Neural Regen Res. 2016;11(2): 285-291.
[50] Sands KA, Shahripour RB, Kumar G, et al. Acute Isolated Central Facial Palsy as Manifestation of Middle Cerebral Artery Ischemia. J Neuroimaging. 2016.