Biocompatibility evaluation for skin reproducing membrane of medical fibroin

doi:10.3969/j.issn.2095-4344.2016.25.001

Abstract

Abstract:

BACKGROUND: Skin reproducing membrane is a biomaterial used directly in contact with skin defects, so its toxicity to the body must be taken into consideration.

OBJECTIVE: To systematically evaluate the biocompatibility of skin reproducing membrane of medical fibroin.

METHODS: Skin irritation test: the skin defect of New Zealand white rabbit was covered with the skin reproducing membrane of medical fibroin, formaldehyde or normal saline, respectively, and erythema and edema were observed at 24 and 72 hours after treatment. Acute systematic toxicity test: the mice were given extracts of skin reproducing membrane of medical fibroin, phenol and normal saline via tail vein injection; then status of mice, toxicity grade and death were recorded at 24, 48 and 72 hours after injection. Cytotoxicity test: L-929 cells were co-cultured with 100%, 50%, 25%, 10% skin reproducing membrane of medical fibroin extracts, and absorbance values were detected by ultraviolet spectrophotometry at 2, 4 and 7 days after culture, as well as by MTT assay after 48 hours of culture, respectively. Besides, L-929 cells were co-cultured with 100% skin reproducing membrane of medical fibroin extracts, and mRNA expression of fibronectin was determined by qPCR technology after 48-hour culture.

RESULTS AND CONCLUSION: Skin reproducing membrane of medical fibroin has no adverse reaction, acute cytotoxicity, skin irritation and systemic toxicity, and additionally, it does not affect mRNA expression of fibronectin. In general, skin reproducing membrane of medical fibroin has good biocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Skin, Cytotoxicity Tests, Immunologic, Silk, Tissue Engineering

CLC Number:

R318

Cao Li-nan, Xu Bao-lai. Biocompatibility evaluation for skin reproducing membrane of medical fibroin[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3653-3658.

Figures/Tables 4

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