Subcutaneous injection of basic fibroblast growth factors promotes the proliferation of hippocampal neural stem cells in rats with vascular dementia

doi:10.3969/j.issn.2095-4344.2016.23.019

Abstract

Abstract:

BACKGROUND: As a neurotrophic factor, basic fibroblast growth factors extensively distribute in the central nervous system, and play an important physiological role by combination with their relative receptors.
OBJECTIVE: To investigate the effect of basic fibroblast growth factors on the learning ability and proliferation of nestin-positive hippocampal neural stem cells in rats with vascular dementia.
METHODS: Totally 30 Sprague-Dawley rats were randomly divided into vascular dementia, sham operation and treatment groups. The vascular dementia and treatment groups were for preparing vascular dementia model, and the treatment group was given subcutaneous injection of basic fibroblast growth factors. Subsequently, at 4 weeks, the learning ability of rats and the number of nestin-positive hippocampal neural stem cells was detected by the Morris water maze test and immunohistochemical staining, respectively.
RESULTS AND CONCLUSION: Compared with the vascular dementia group the latency period was significantly shorter in the sham operation and treatment groups, and the number of times crossing the target quadrant was significantly higher in the sham operation and treatment groups (P < 0.05). But there was no significant difference between the sham operation and treatment groups (P > 0.05). Under fluorescence microscope, yellow-green fluorescence stained neurons positive for basic fibroblast growth factor could be found in the CA1, CA2 and CA3 of the treatment group. Additionally, the number of nestin-positive neurons in the CA1 area of the hippocampus was the most in the treatment group, followed by the sham operation group, and the least in the vascular dementia group. These results suggest that the subcutaneous injection of basic fibroblast growth factors can migrate to the hippocampus, , and improve the learning ability of rats by inducing proliferation of nestin-positive hippocampal neural stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Dementia, Vascular, Fibroblast Growth Factor 2, Neural Stem Cells, Tissue Engineering

CLC Number:

R394.2

Liu Sen, Dai Zhen-xia, Wu Chun-fang, Hu Jing, Cui Bei . Subcutaneous injection of basic fibroblast growth factors promotes the proliferation of hippocampal neural stem cells in rats with vascular dementia[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(23): 3470-3475.

Figures/Tables 2

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