Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (23): 3476-3483.doi: 10.3969/j.issn.2095-4344.2016.23.020

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Basic fibroblast growth factors promote proliferation of endogenous neural stem cells after spinal cord injury in mice

Li Yu-bo1, Ding Li-xiang2   

  1. 1Department of Orthopedics, Affiliated Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China
    2Department of Spine Surgery, Affiliated Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China
  • Received:2016-04-04 Online:2016-06-03 Published:2016-06-03
  • Contact: Li Yu-bo, Department of Orthopedics, Affiliated Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China
  • About author:Li Yu-bo, Master, Physician, Department of Orthopedics, Affiliated Beijing Chaoyang Hospital of Capital Medical University, Beijing 100020, China
  • Supported by:

    the Youth Project of National Natural Science Foundation of China, No. 31300923

Abstract:

BACKGROUND: So far steroid pulse therapy and surgical decompression are the main accepted therapies for spinal cord injury, but these methods make no effects on injured neurons. Nerve growth factors and endogenous neural stem cells play a role in the neuronal regeneration and remyelination, which provides a new idea for spinal cord injury treatment.
OBJECTIVE: To explore the effect of the basic fibroblast growth factor on proliferation of endogenous neural stem cells after spinal cord injury and to analyze its relationship with the increased fluoro-gold labeled neurons.
METHODS: Totally 48 Kunming mice were randomly divided into four groups including normal, spinal cord injury, treatment and sham operation groups. Acute spinal cord injury models were established in the spinal cord injury and treatment groups, and the normal group was subjected to operation that did not damage the spinal cord. At 2 hours after regaining consciousness, the treatment group was given daily injection of MTPBS containing 25 μg/kg basic fibroblast growth factors and 1% album. And at 7 days, laminectomy was carried out again at the L1 segment in the former three groups and a small piece of sterile gelfoam soaked with fluoro-gold was inserted into the incision. The sham operation group was given no processing. Afterwards, mouse motor behavior was assessed using Rotarod and Platform Hang tests; neurons in the corticospinal and rubrospinal tracts were labeled with fluoro-gold; the number of endogenous neural stem cells positive for nestin was detected by immunohistochemistry method. Besides, the correlation between the number of fluoro-gold labeled neurons and the number of endogenous neural stem cells was assessed.
RESULTS AND CONCLUSION: The basic fibroblast growth factor could significantly improve the mouse motor behavior after spinal cord injury. And the number of endogenous neural stem cells was significantly increased after the basic fibroblast growth factor injection, which was related to the increased fluoro-gold labeled neurons. In conclusion, basic fibroblast growth factors play an important role in the proliferation of endogenous neural stem cells after spinal cord injury. Furthermore, endogenous neural stem cells improve locomotive behaviors by encouraging the neuronal proliferation.

 

 中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Spinal Cord Injuries, Fibroblast Growth Factor 2, Neural Stem Cells, Tissue Engineering

CLC Number: