Emodin effects on renal ischemia/reperfusion injury after bone marrow mesenchymal stem cell transplantation

doi:10.3969/j.issn.2095-4344.2016.14.011

Abstract

Abstract:

BACKGROUND: Studies have shown that emodin protects against intestinal ischemia-reperfusion injury by inhibiting the release of inflammatory factors.

OBJECTIVE: To investigate the effect of emodin on renal ischemia/reperfusion injury after bone marrow mesenchymal stem cell transplantation.

METHODS: Forty Sprague-Dawley rats were randomized into five groups (n=8 per group): sham group, model group (renal ischemia/reperfusion injury group), bone marrow mesenchymal stem cell group (cell transplantation group), low-dose emodin group, high-dose emodin group. Rats in the latter four groups were pretreated with or without different concentrations (30 and 60 mg/kg) of emodin for 7 days, and then were subjected to clamping bilateral renal pedicles for 45 minutes, followed by injection of 1 mL bone marrow mesenchymal stem cell suspension. Six hours after reperfusion, the pathological changes of renal tissues were examined by hematoxylin-eosin staining; the mRNA levels of tumor necrosis factor α, interleukin-6, interleukin-18, TLR2 and TLR4 detected by real-time fluorescence quantitative PCR; and the protein expression of COX-2, ICAM-1 and iNOS determined by western blot.

RESULTS AND CONCLUSION: Compared with the sham group, rats in the model group showed obvious features of severe acute tubular damage and inflammatory cell infiltration. In the cell transplantation group, tubular epitheliael cells were partially lost with some inflammatory cells infiltrated in the renal interstitium. In the emodin groups, the tubular lumen was practically intact with little renal interstitial inflammatory cells. Compared with the sham group, a significant increase in the mRNA levels of tumor necrosis factor α, interleukin-6, interleukin-18, TLR2 and TLR4 as well as in the protein levels of COX-2, ICAM-1 and iNOS was found in the model group (both P < 0.05). However, bone marrow mesenchymal stem cell transplantation attenuated this ischemia/reperfusion-induced increase in the expression of the above-mentioned factors (P < 0.05). Furthermore, the effects of bone marrow mesenchymal stem cell transplantation were further augmented by pretreatment with emodin in a dose-dependent manner. These findings suggest that emodin can enhance the protective effects of bone marrow mesenchymal stem cells on renal ischemia/reperfusion injury.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Reperfusion Injury, Kidney, Emodin, Tissue Engineering

Li Zhong. Emodin effects on renal ischemia/reperfusion injury after bone marrow mesenchymal stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2052-2058.

Figures/Tables 4

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