Directional and specific characteristics of astrocyte fiber growth and synapse formation

doi:10.3969/j.issn.2095-4344.2015.42.012

Abstract

Abstract:

BACKGROUND: Studies have shown that astrocytes participate not only in the passive metabolic and structural support, but also actively in the central nervous system information processing.

OBJECTIVE: By observing the directionality and specificity of astrocyte fiber growth and synapse formation, to explore the possible mechanisms of astrocytes and neurons in the interaction.

METHODS: Primary cells from the hippocampi of green fluorescent protein transgenic newborn mice were isolated and cultured, glial fibrillary acidic protein was used as specific marker for astrocytes in immunofluorescence staining, and then the results were observed and scanned by confocal microscope.

RESULTS AND CONCLUSION: The elongation of astrocyte fibers was directional and specific, and terminated to the pyramidal cells nearby. The end of astrocyte fibers and pyramidal cells contributed to the synapse formation. However, not all the projections were toward the adjacent cells, and synapse-like structure was not only formed nearby. The results implicated that both astrocytes fiber elongation and synapse formation have the directionality and specificity, providing evidence for further studies on signal transduction between astrocytes and pyramidal cells.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Astrocytes, Synapses, Signal Transduction, Green Fluorescent Proteins

Cao Jing-li, Li Qi, Wang Ya-jie . Directional and specific characteristics of astrocyte fiber growth and synapse formation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(42): 6787-6792.

Figures/Tables 1

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