OBJECTIVE: To investigate the histocompatibility and osteogenic induction of geneX artificial bone combined with bone marrow mesenchymal stem cells and transforming growth factor beta 2 (TGF-β2).

METHODS: Injectable geneX was co-cultured with bone marrow mesenchymal stem cells for 5 days, and under the electron microscope, the histocompatibility of the artificial bone was observed. Passage 3 rabbit bone marrow mesenchymal stem cells were divided into three groups: simple cell group cultured with osteogenic medium, cell scaffold group cultured with geneX+osteogenic medium, combined group cultured with geneX+TGF-β2+ osteogenic medium. After 7, 14, 21 days, cell morphology, alkaline phosphatase activity detection, MTT detection, methyl thymol blue detection and alizarin red staining were performed.

RESULTS AND CONCLUSION: After osteogenic induction, bone marrow mesenchymal stem cells were fibroblast-like cells and adhered to the surface of geneX bone with strong secretion of extracellular matrix. Cell proliferation and osteogenic activity in the combined group were stronger than those in the simple cell and cell scaffold groups (P < 0.05). The alkaline phosphatase activity was also higher in the combined group than the other two groups. These findings indicate that the geneX/bone marrow mesenchymal stem cells/TGF-β2 composite has good histocompatibility and pro-osteogenic differentiation ability.

 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程