Raman spectroscopy of bone marrow mesenchymal stem cells in medium-frequency pulsed electromagnetic fields

doi:10.3969/j.issn.2095-4344.2014.37.005

Abstract

Abstract:

BACKGROUND: Studies about low-frequency pulsed electromagnetic fields interfering with bone marrow mesenchymal stem cells proliferation and differentiation are many, but the Raman spectra of single stem cells irradiated in electromagnetic fields analyzed by surface Raman spectroscopy analysis are rarely reported.
OBJECTIVE: To compare the difference in Raman spectra of bone marrow mesenchymal stem cells with or with no irradiation of 3 000 Hz pulsed electromagnetic fields.
METHODS: Bone marrow mesenchymal stem cells isolated from Sprague-Dawley rats were cultured and identified. Passage 3 cells were inoculated into 6-well plates and divided into two groups: pulsed electromagnetic field irradiation group and blank control group. After cultured for 7 days, cells in the two groups were transferred to physiological saline, and 30 cells were randomly collected from each group. Four Raman spectra were harvested from each cell and the average relative intensity of Raman spectra was calculated and compared between two groups.
RESULTS AND CONCLUSION: There were the same Raman peaks in the two groups, and the waveforms were basically same in the two group based on the curve mapping by origin 7.0 software. The peak value in the irradiation group was decreased compared with the blank control group. Laser optical tweezers Raman spectroscopy can be applied to study the biochemical changes of a single stem cell at the molecular level. The Raman spectra of bone marrow mesenchymal stem cells irradiated by 3 000 Hz pulsed electromagnetic fields differ from those without irradiation, and the peak also lowered after irradiation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, electromagnetic fields, optical tweezers

CLC Number:

R394.2

Cui Xiang-rong, Su Wei, Wu Zhi-hui, Meng Ling-jing, Huang Zhao, Qin Wan-an. Raman spectroscopy of bone marrow mesenchymal stem cells in medium-frequency pulsed electromagnetic fields[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(37): 5929-5934.

Figures/Tables 2

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