Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (34): 5413-5417.doi: 10.3969/j.issn.2095-4344.2014.34.001
Geng Hai-xia, Guo Xiu-juan, Qian Jun-rong, Feng Wei
Revised:
2014-07-16
Online:
2014-08-20
Published:
2014-08-20
Contact:
Geng Hai-xia, Master, Chief physician, Professor, Department of Stomatology, Affiliated Hospital of Jining Medical College, Jining 272000, Shandong Province, China
About author:
Geng Hai-xia, Master, Chief physician, Professor, Department of Stomatology, Affiliated Hospital of Jining Medical College, Jining 272000, Shandong Province, China
Supported by:
the Natural Science Foundation of Shandong Province, No. ZR2010HL046; the Scientific Development Plan of Shandong Health Bureau, No. 2011HZ018
CLC Number:
Geng Hai-xia, Guo Xiu-juan, Qian Jun-rong, Feng Wei . Imaging evaluation of hydroxyapatite/gel nano-composite in rabbit skull defect repair[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(34): 5413-5417.
2.1 实验动物数量分析 实验兔共15只,无感染和死亡,均纳入结果分析。 2.2 羟基磷灰石/凝胶纳米复合物植入后兔颅骨缺损区的一般形态 所有兔在植入后1 h内即可苏醒,植入后1 d后,精神状态有所恢复,均可自主进食,全部实验周期内实验动物无死亡,生长良好,均存活至取材。颅骨取材区及植骨区伤口均为一期愈合,植入物无脱出,无异物及免疫排斥反应。 图2是植入后12周时取材后带修复材料骨块,可以观察到,自体颅骨修复可见缺损区由致密的骨组织修复,且与周围骨组织无明显界限,羟基磷灰石/凝胶纳米复合物新型仿生复合物中央部位颜色偏黄、硬度偏低,材料表面可见有较多骨痂形成并向中央集中,其周边与骨断端之间已有较牢固的骨性连接形成,扪之有一定的强度,与周围正常骨组织也已经无明显界限。 2.3 羟基磷灰石/凝胶纳米复合物植入后兔颅骨缺损区的X射线观察结果 植入后8周:大鼠自体颅骨修复区与周围正常的骨组织形态类似;羟基磷灰石/凝胶纳米复合物修复区中央位置骨质致密,形态接近正常骨组织,边缘略显模糊,见图3A。"
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