Protective effects of piperine on alveolar bone and collagen in a periodontitis model

doi:10.3969/j.issn.2095-4344.2016.40.016

Abstract

Abstract:

BACKGROUND: Piperine in models of pancreatitis, gout, middle cerebral artery infarction has anti-inflammatory, antioxidant and immune regulatory effects, but its effects on periodontitis model are not clear.

OBJECTIVE: To observe the protective effect of piperine on bone absorption and degradation of collagen in experimental rat models of periodontitis.

METHODS: Rat models of periodontitis were established by ligaturing the dental cervix of rat mandibular first molar with 3-0 silk. On day 1 before model establishment, rats were intragastrically administered piperine 50 and 100 mg/kg. There were healthy control group and model group. Related detection was performed 8 weeks after model establishment.

RESULTS AND CONCLUSION: (1) Results of quantitative CT analysis: compared with the healthy control group, the distance from the first molar enamelo-cemental junction to the alveolar ridge crest was significantly lower in the model group (P < 0.05). The degree of alveolar damage was significantly improved in the 50 and 100 mg/kg piperine groups compared with the model group (P < 0.05). (2) Related factor protein expression: compared with the model group, matrix metalloproteinase-8, -13 and interleukin-1β protein expression was significantly decreased in the 100 mg/kg piperine group (P < 0.05); matrix metalloproteinase-8 protein expression was significantly decreased in the 50 mg/kg piperine group (P < 0.05). (3) Collagen fiber morphology: compared with the model group, collagen fibers arranged orderly and collagen fiber area significantly increased in the 50 and 100 mg/kg piperine groups (P < 0.05). (4) Results confirmed that piperine could reduce the alveolar bone resorption, reduce the degradation of collagen fibers and protect the periodontal tissues in models of periodontitis. Its mechanism is associated with the inhibition of matrix metalloproteinase-8, -13 and interleukin-1β protein expression.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Periodontitis, Models, Animal

CLC Number:

R318

Ge Run, Yang Lan. Protective effects of piperine on alveolar bone and collagen in a periodontitis model[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(40): 6034-6040.

Figures/Tables 2

2.1 造模成功动物数量及过程纳入大鼠32只均进入结果分析。造模动物流程表见图1。 2.2 模型创新性锐分离大鼠双侧下颌第一磨牙颊侧牙龈、局部黏结牙周病患者牙石后，龈沟内滴入牙周病患者唾液的方法，并辅以高糖黏性食物饲养，成功制备了牙周炎动物模型。 2.3 模型稳定性局部黏结牙石加混合细菌感染的方法建立的大鼠牙周炎模型可以模拟人类牙周炎的组织变化，是一种接近于人类临床的简单、实惠、有效的建模方法。 2.4 牙周炎模型大鼠体质量变化实验前，4组大鼠的体质量差异无显著性意义(P=0.333)。丝线结扎后第7天，健康对照组大鼠体质量为(245.1±14.1) g，模型组体质量为(237.9±13.7) g。胡椒碱治疗组的体质量：胡椒碱50 mg/kg组为(244.1±15.1) g；胡椒碱100 mg/kg组为(231.6± 15.7g)，与模型组相比差异无显著性意义(P >0.05)。 2.5 胡椒碱对骨吸收的保护作用健康对照组可见牙槽骨完整，骨小梁排列整齐，无破坏；模型组可见牙槽骨吸收严重，裸露牙根，骨小梁破坏严重；胡椒碱 50 mg/kg组和胡椒碱100 mg/kg组组与模型组相比较，牙槽骨吸收减轻，骨小梁破坏减轻，见图2。大鼠第一磨牙釉牙骨质界到牙槽嵴顶的距离显著性降低。胡椒碱各剂量组的大鼠第一磨牙釉牙骨质界到牙槽嵴顶的距离均明显低于模型组(P < 0.05)。见图3。 2.6 胡椒碱对牙周炎模型大鼠牙周组织学变化的影响健康对照组牙周组织完整；模型组牙周组织可见大量的炎症细胞浸润；胡椒碱50，100 mg/kg组牙周组织炎症细胞浸润明显减轻，水肿少。见图4。 2.7 胡椒碱对牙周炎模型大鼠牙周组织胶原纤维的影响建模后8周，检测各组牙周组织各自的胶原纤维的分布和降解情况。胶原纤维在图中显示为黄色、绿色、红色。在健康对照组大鼠牙周组织中，胶原纤维分布均匀、致密，排列整齐，胶原纤维方向较一致，未见明显空洞，且边界较完整，未见毛边。与健康对照组比较，模型组中胶原纤分布不均匀、疏松，排列紊乱，胶原纤维之间可见黑色吸收空洞，胶原纤维面积明显下降(P < 0.05)。胡椒碱50，100 mg/kg组胶原纤维面积明显比模型组大，纤维内空洞较少，排列较均匀，两组之间也差异有显著性意义(P < 0.05)。见图5，6。 2.8 胡椒碱对牙周组织基质金属蛋白酶8、基质金属蛋白酶13、EMMPRIN、肿瘤坏死因子α和白细胞介素1β蛋白表达的影响建模后8周，Western blot检测显示，与健康对照组相比，模型组大鼠基质金属蛋白酶8、基质金属蛋白酶13、EMMPRIN、肿瘤坏死因子α和白细胞介素1β蛋白表达增高(P < 0.05)。与模型组相比，胡椒碱100 mg/kg组基质金属蛋白酶8、基质金属蛋白酶13和白细胞介素1β蛋白表达下降，胡椒碱50 mg/kg组基质金属蛋白酶8蛋白表达下降，但对基质金属蛋白酶13和白细胞介素1β蛋白表达则无明显作用。另外，两种剂量胡椒碱对EMMPRIN和肿瘤坏死因子α蛋白均无明显作用(图7，表1)。"

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