Establishment of a dog model of pyogenic spinal infection

doi:10.3969/j.issn.2095-4344.2016.40.013

Abstract

Abstract:

BACKGROUND: Spine infection models are rarely reported in the literature. There are fewer reports on the imaging and histological observations of the animal model of spinal infection.

OBJECTIVE: To develop a canine model of pyogenic spinal infection.

METHODS: Twelve Chinese dogs were adopted in the study. The dogs were in the right arm recumbent. The surgery was conducted by retroperitoneal approach to expose T_12/L₁ intervertebral space. The intervertebral discs that exposed the intervertebral space were partially removed, and the end plate of the adjacent two vertebrae was deliberately curetted out. Suspension with different concentrations of Staphylococcus aureus and sodium morrhuate were injected into the dog intervertebral space. The aim of removal of partial intervertebral discs and destruction of endplate was to make the vertebral body bone better in contact with the bacteria. The same operation was conducted in L_5/6 clearance of the animal spine. At 14 days after surgery, animals were sacrificed. The infected intervertebral disc and adjacent vertebral bodies were taken out. Bacterial contents of specimens were quantitatively determined. During sacrificing, partial liver tissues and blood samples were collected for bacterial culture. The occurrence of systemic infection was observed.

RESULTS AND CONCLUSION: (1) Spondylodiscitis of the lumbar spinal column was consistently produced in 90% (9/10) the sites challenged with 10² colony-forming units (CFU) Staphylococcus aureus. Liver biopsy and blood culture did not show any signs of systematic infections. (2) Two out of four animals implanted the suspension with a concentration higher than 10³ CFUs died within 3 days post-implantation. (3) These results suggested that a new canine model of pyogenic spinal infection was developed. This model could be used to test the efficacy of different anti-infection strategies.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Models, Animal, Infection, Staphylococcus aureus, Tissue Engineering

CLC Number:

R318

Chen Wei-hua, Lv Guo-hua, Zhou Bin, Kang Yi-jun. Establishment of a dog model of pyogenic spinal infection[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(40): 6014-6020.

Figures/Tables 2

References

[1] Belzunegui J, Intxausti JJ, De Dios JR, et al. Haematogenous vertebral osteomyelitis in the elderly. Clin Rheumatol. 2000;19(5):344-347.
[2] Conaughty JM, Chen J, Martinez OV, et al. Efficacy of linezolid versus vancomycin in the treatment of methicillin-resistant Staphylococcus aureus discitis: a controlled animal model. Spine. 2006;31(22): E830-832.
[3] Pupaibool J, Vasoo S, Erwin PJ, et al. The utility of image-guided percutaneous needle aspiration biopsy for the diagnosis of spontaneous vertebral osteomyelitis: a systematic review and meta-analysis. Spine J. 2015; 15(1):122-131.
[4] Weissman S, Parker RD, Siddiqui W, et al. Vertebral osteomyelitis: retrospective review of 11 years of experience. Scand J Infect Dis. 2014; 46(3):193-199.
[5] Cheung WY, Luk KD. Pyogenic spondylitis. Int Orthop. 2012;36(2):397-404.
[6] Talia AJ, Wong ML, Lau HC, et al. Safety of instrumentation and fusion at the time of surgical debridement for spinal infection. J Clin Neurosci. 2015; 22(7):1111-1116.
[7] Zimmerli W. Clinical practice. Vertebral osteomyelitis. N Engl J Med. 2010;362(11):1022-1029.
[8] Park KH, Cho OH, Lee YM, et al. Therapeutic outcomes of hematogenous vertebral osteomyelitis with instrumented surgery. Clin Infect Dis. 2015;60(9): 1330-1338.
[9] Mohamed AS, Yoo J, Hart R, et al. Posterior fixation without debridement for vertebral body osteomyelitis and discitis. Neurosurg Focus. 2014; 37(2):E6.
[10] Chung TC, Yang SC, Chen HS, et al. Single-stage anterior debridement and fibular allograft implantation followed by posterior instrumentation for complicated infectious spondylitis: report of 20 cases and review of the literature. Medicine (Baltimore). 2014;93(27):e190.
[11] Bydon M, De la Garza-Ramos R, Macki M, et al. Spinal instrumentation in patients with primary spinal infections does not lead to greater recurrent infection rates: an analysis of 118 cases. World Neurosurg. 2014; 82(6):e807-814.
[12] Nijhof MW, Fleer A, Hardus K, et al. Tobramycin- containing bone cement and systemic cefazolin in a one-stage revision. Treatment of infection in a rabbit model. J Biomed Mater Res. 2001;58(6):747-753.
[13] Craig MR, Poelstra KA, Sherrell JC, et al. A novel total knee arthroplasty infection model in rabbits. J Orthop Res. 2005;23(5):1100-1104.
[14] van der Borden AJ, van der Mei HC, Busscher HJ. Electric block current induced detachment from surgical stainless steel and decreased viability of Staphylococcus epidermidis. Biomaterials. 2005; 26(33): 6731-6735.
[15] Lucke M, Wildemann B, Sadoni S, et al. Systemic versus local application of gentamicin in prophylaxis of implant-related osteomyelitis in a rat model. Bone. 2005;36(5):770-778.
[16] Carmen JC, Roeder BL, Nelson JL, et al. Treatment of biofilm infections on implants with low-frequency ultrasound and antibiotics. Am J Infect Control. 2005; 33(2):78-82.
[17] Sheehan E, McKenna J, Mulhall KJ, et al. Adhesion of Staphylococcus to orthopaedic metals, an in vivo study. J Orthop Res. 2004; 22(1):39-43.
[18] Huneault LM, Lussier B, Dubreuil P, et al. Prevention and treatment of experimental osteomyelitis in dogs with ciprofloxacin-loaded crosslinked high amylose starch implants. J Orthop Res. 2004;22(6):1351-1357.
[19] Yin LY, Lazzarini L, Li F, et al. Comparative evaluation of tigecycline and vancomycin, with and without rifampicin, in the treatment of methicillin-resistant Staphylococcus aureus experimental osteomyelitis in a rabbit model. J Antimicrob Chemother. 2005;55(6): 995-1002.
[20] Fukushima N, Yokoyama K, Sasahara T, et al., Establishment of rat model of acute staphylococcal osteomyelitis: relationship between inoculation dose and development of osteomyelitis. Arch Orthop Trauma Surg. 2005;125(3):169-176.
[21] Faber C, Hoogendoorn RJ, Stallmann HP, et al. In vivo comparison of Dhvar-5 and gentamicin in an MRSA osteomyelitis prevention model. J Antimicrob Chemother. 2004;54(6):1078-1084.
[22] Makinen TJ, Veiranto M, Knuuti J, et al. Efficacy of bioabsorbable antibiotic containing bone screw in the prevention of biomaterial-related infection due to Staphylococcus aureus. Bone. 2005;36(2):292-299.
[23] Joosten U, Joist A, Gosheger G, et al. Effectiveness of hydroxyapatite-vancomycin bone cement in the treatment of Staphylococcus aureus induced chronic osteomyelitis. Biomaterials. 2005;26(25):5251-5258.
[24] Poelstra KA, Barekzi NA, Grainger DW, et al. A novel spinal implant infection model in rabbits. Spine. 2000; 25(4):406-410.
[25] An YH, Kang QK, Arciola CR. Animal models of osteomyelitis. Int J Artif Organs. 2006;29(4):407-420.
[26] Korovessis P, Petsinis G, Koureas G, et al. One-stage Combined Surgery with Mesh Cages for Treatment of Septic Spondylitis. J.Clin Orthop Relat Res. 2006; 444:51-59.
[27] Hadjipavlou AG, Mader JT, Necessary JT, et al. Hematogenous pyogenic spinal infections and their surgical management. Spine. 2000;25(13):1668-1679.
[28] Garg V, Kosmas C, Young PC, et al. Computed tomography-guided percutaneous biopsy for vertebral osteomyelitis: a department's experience. J.Neurosurg Focus. 2014;37(2):E10.
[29] Osenbach RK, Hitchon PW, Menezes AH. Diagnosis and management of pyogenic vertebral osteomyelitis in adults. Surg Neurol. 1990;33(4):266-275.
[30] Rissing JP, Buxton TB, Weinstein RS, et al. Model of experimental chronic osteomyelitis in rats. Infect Immun. 1985;47(3):581-586.
[31] Mayberry-Carson KJ, Tober-Meyer B, Smith JK, et al. Bacterial adherence and glycocalyx formation in osteomyelitis experimentally induced with Staphylococcus aureus. Infect Immun. 1984;43(3): 825-833.
[32] Norden CW, Budinsky A. Treatment of experimental chronic osteomyelitis due to Staphylococcus aureus with ampicillin/sulbactam. J Infect Dis. 1990;161(1): 52-53.
[33] Norden CW, Keleti E. Experimental osteomyelitis caused by Pseudomonas aeruginosa. J Infect Dis. 1980;141(1):71-75.
[34] Stroncek DF, Hutton SW, Silvis SE, et al. Sodium morrhuate stimulates granulocytes and damages erythrocytes and endothelial cells: probable mechanism of an adverse reaction during sclerotherapy. J Lab Clin Med. 1985; 106(5):498-504.
[35] Dekel S, Francis MJ. The treatment of osteomyelitis of the tibia with sodium salicylate. An experimental study in rabbits. J.J Bone Joint Surg Br. 1981;63-B(2): 178-184.
[36] Rissing JP, Buxton TB, Fisher J, et al. Arachidonic acid facilitates experimental chronic osteomyelitis in rats. Infect Immun. 1985;49(1):141-144.