Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (40): 6048-6053.doi: 10.3969/j.issn.2095-4344.2016.40.018

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Establishment and evaluation of a rhesus monkey model of experimental type 2 diabetes mellitus

Song Qiao-qiao1, Zhou Hui-liang2, Zhen Hai-tao1, Wang Na1, Deng Jing1, Wang Jin-xiang3, Pan Xing-hua3   

  1. 1Department of Internal Medicine, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China; 2Xianning Central Hospital, Xianning 437100, Hubei Province, China; 3Stem Cell Engineering Laboratory of Yunnan Province, Stem Cells and Organ Tissue Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China
  • Revised:2016-07-05 Online:2016-09-30 Published:2016-09-30
  • Contact: Pan Xing-hua, M.D., Professor, Stem Cell Engineering Laboratory of Yunnan Province, Stem Cells and Organ Tissue Engineering Research Center, Kunming General Hospital of Chengdu Military Command, Kunming 650032, Yunnan Province, China
  • About author:Song Qiao-qiao, Master, Teaching assistant, Department of Internal Medicine, Hubei University of Science and Technology, Xianning 437100, Hubei Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31172170; a grant from Hubei University of Science and Technology, No. KY13065

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Abstract:

BACKGROUND: At present, there are few reports about the non-human primate models of type 2 diabetes mellitus in domestic and abroad, so it lacks of standardized production methods and evaluation criteria.

OBJECTIVE: To establish a safe and effective type 2 diabetes mellitus model of rhesus monkey and evaluation method.
METHODS: Twelve rhesus monkeys were randomly assigned to experimental group (n=9) and control group (n=3). Rhesus monkeys in the experimental group were fed with high-glucose and high-fat diet for 4 weeks, and intraperitoneally injected with 30 mg/kg streptozotocin to establish models of type 2 diabetes mellitus. Rhesus monkeys in the control group were fed with an equal volume of physiological saline. At 12 weeks after injection, peripheral blood serum was collected to measure fasting blood glucose, lipids, insulin, and C-peptide levels. Intravenous glucose tolerance test and C-peptide release test were used to detect pancreatic gland and pancreatic islet function. Histopathological examination was performed in pancreas, kidney and liver.

RESULTS AND CONCLUSION: (1) 12 weeks after injection, fasting blood glucose, triglycerides, and total cholesterol levels were significantly higher in the experimental group than in the control group (P < 0.05). Insulin and C-peptide levels were significantly lower in the experimental group than in the control group  (P < 0.05). (2) The area under the curve for intravenous glucose tolerance test was increased in the experimental group than in the control group (P < 0.05). The area under the curve for C-peptide response test was significantly reduced in the experimental group than in the control group (P < 0.05). (3) The pathological sections of pancreas, kidney and liver showed typical pathological changes of diabetes in the experimental group. (4) It is confirmed that we got high achievement about rhesus monkey models of type 2 diabetes mellitus made by high-glucose and high-fat diet combined with low-dose streptozotocin. It is a feasible, safe and effective method. 

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Macaca, Diabetes Mellitus, Type 2, Models, Animal, Tissue Engineering

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