Chinese Journal of Tissue Engineering Research ›› 2014, Vol. 18 ›› Issue (21): 3361-3366.doi: 10.3969/j.issn.2095-4344.2014.21.015
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Chang Rui, Yin Xiao-long, Shang Bao-sheng, He Peng
Online:
2014-05-21
Published:
2014-05-21
Contact:
Chang Rui, Master, Associate chief physician, Department of Orthopedics, Hospital of China XD Group, Xi’an 710077, Shaanxi Province, China
About author:
Chang Rui, Master, Associate chief physician, Department of Orthopedics, Hospital of China XD Group, Xi’an 710077, Shaanxi Province, China
Supported by:
the Project of Shaanxi Provincial Health Bureau in 2012, No. 2012D56
CLC Number:
Chang Rui, Yin Xiao-long, Shang Bao-sheng, He Peng . Combination of olfactory ensheathing cells and chitosan in treatment of peripheral nerve injury[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(21): 3361-3366.
2.3 嗅鞘细胞复合壳聚糖支架修复坐骨神经损伤大鼠一般形态变化 各组大鼠建模后大体观察所有大鼠均正常存活,各组均出现足肿胀,但无足底溃疡、化脓或感染。表明细胞移植不会引起排斥反应,安全良好。建模后1 d,各组SD大鼠患肢足趾弯曲,无法伸展,行走时拖拽,针刺无收缩反应。建模后2周,除空白单纯壳聚糖支架组外,其他2组SD大鼠肌肉萎缩有恢复,行走时拖曳程度降低,针刺能引起微弱的收缩。修复后4周,除空白单纯壳聚糖支架组外,其他2组大鼠肌肉萎缩恢复,僵直消失,其中嗅鞘细胞复合壳聚糖支架组的大鼠足趾有较大握力,存在明显针刺反应。 2.4 嗅鞘细胞复合壳聚糖支架修复后大鼠坐骨神经功能变化 表1显示建模后1-4周各组大鼠坐骨神经指数变化。建模后1周,各组坐骨神经功能指数大致相同,约为-83.6,1周后各组大鼠的坐骨神经功能指数均随时间的延长而提高。修复后4周,嗅鞘细胞复合壳聚糖支架组修复速率最快,3组间坐骨神经功能指数比较均差异有显著性意义(P < 0.05)。"
2.6 嗅鞘细胞复合壳聚糖支架修复后大鼠坐骨神经组织学形态 苏木精-伊红染色显示,修复后4周,坐骨神经组织纵切面光学显微镜可见,嗅鞘细胞复合壳聚糖支架组有新生的神经达到远侧端,且再生神经周围没有炎症反应,证明壳聚糖支架具有良好的组织相容性,见图3A。空白单纯壳聚糖支架组神经束内大部分空间被纤维组织填充,仅见少量神经纤维,见图3B。单纯壳聚糖支架组新生神经干数目明显多于空白单纯壳聚糖支架组,但是神经束排列没有规律,密度较小,见图3C。扫描电镜下显示,嗅鞘细胞复合壳聚糖支架组嗅鞘细胞与支架复合情况显示,材料的内部结构疏松排列,多孔结构规则有序,说明嗅鞘细胞与支架复合贴合良好,见图3D。 2.7 移植后生物相容性及不良反应评价 嗅鞘细胞复合壳聚糖支架修复坐骨神经损伤大鼠生物相容性良好,无不良反应发生。"
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