Effect of Breviscapine on the survival and growth of rat cortical neurons cultured in vitro

doi:10.3969/j.issn.2095-4344.2014.20.015

Abstract

Abstract:

BACKGROUND: Nerve growth factor plays an irreplaceable role on the survival, differentiation and maintenance of the central nervous system cells, also promote the growth and development of these cells.
OBJECTIVE: To explore effect of Breviscapine on the growth of in vitro cultured neurons in cerebral cortex of Sprague-Dawley rats, and preliminarily investigate the action mechanism.
METHODS: The cerebral cortex of newborn Sprague-Dawley rat embryos was collected, the neurons were primary cultured and randomly divided into three groups: normal control group (no treatment), control group (neurons were cultured with normal saline), Breviscapine group (neurons were cultured with 10 g/L Breviscapine). Furthermore each group was assigned into 24-hour and 48-hour subgroups. Images were captured from the 24-well plates at each time points. RT-PCR was applied to examine the expression of nerve growth factor and TrkA mRNA. MTT was used to detect the neuronal growth at each time point.
RESULTS AND CONCLUSION: There was no difference between normal control group and control group in the cell counts, cell body area, neurite length and viability (P > 0.05), as the time prolonged, all data were raised in the
two groups (P < 0.05). Breviscapine group showed a higher cell count, cell body area, neurite length and viability than normal control group and control group (P < 0.05). No significant difference was found in the expression of nerve growth factor and TrkA mRNA between normal control group and control group (P > 0.05). At each time point, these data in Breviscapine group were increased compared with normal control group and control group (P < 0.05). Breviscapine can promote the survival and growth of brain-derived neurons in Sprague-Dawley rats, and the mechanism might depend on the up-regulating expression of nerve growth factor and its receptor TrkA.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: drugs, Chinese herbal, nerve growth factor, receptor, TrkA

CLC Number:

R318

Li Jun-yan, Wang Dong-yan, Yang Jin-wei, Zhao Nan, Li Li-yan, Cheng Jing-ru, Ru Jin, Guo Jian-hui, Liu Jun. Effect of Breviscapine on the survival and growth of rat cortical neurons cultured in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(20): 3198-3201.

Figures/Tables 2

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