Induced differentiation of human umbilical cord mesenchymal stem cells into neural stem cells in vitro

doi:10.3969/j.issn.2095-4344.2014.01.013

Abstract

Abstract:

BACKGROUND: Compared with mesenchymal stem cells from bone marrow and fat, human umbilical cord-derived mesenchymal stem cells, as one of the most potential cell sources to repair the central nervous system, are easy-based, more primitive, and not limited by ethical and legal.
OBJECTIVE: To explore differentiation of human umbilical cord-derived mesenchymal stem cells into neural stem cells induced by the recombinant human basic fibroblast growth factor and recombinant human epidermal growth factor.
METHODS: The human umbilical cord-derived mesenchymal stem cells were induced by basic fibroblast growth factor and epidermal growth factor in vitro. The cell morphology was observed by invert microscope. The
differentiation status was detected by immunofluorescence. The Nestin expression in mRNA level before and after induction was detected by real-time PCR.
RESULTS AND CONCLUSION: The neural stem cell balls were observed after induction. And the Nestin was detected by immunofluorescence and real-time PCR. Nestin could further differentiate to the neuronal markproteins neuron-specific enolase, microtubule-associated protein 2 protein and glial fibrillary acidic protein. Results from this study show that basic fibroblast growth factor and epidermal growth factor can induce human umbilical cord-derived mesenchymal stem cells to differentiate to neural stem cells, neurons and glial cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: stem cells, mesenchymal stem cells, umbilical cord, neural stem cells, epidermal growth factor, fibroblast growth factors

CLC Number:

R394.2

Li Wei-wei, Yao Xing-yu, Yang Li-min, Zhao Peng-wei, Zhang Guo-hua . Induced differentiation of human umbilical cord mesenchymal stem cells into neural stem cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(1): 75-80.

Figures/Tables 4

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