Zebrafish embryonic brain cell apoptosis and c-fos gene expression after hypoxia reperfusion

doi:10.3969/j.issn.2095-4344.2013.37.012

Abstract

Abstract:

BACKGROUND: Foreign scholars have researched hypoxia reperfusion in zebrafish embryos, but there is no research on c-fos gene expression and the mechanism during zebrafish cerebral hypoxia reperfusion.
OBJECTIVE: To observe the zebrafish embryonic brain cell apoptosis and expression of c-fos gene in brain tissues after hypoxia reperfusion.
METHODS: Zebrafish embryos were selected at 48 hours post fertilization. Neonatal hypoxia reperfusion injury was simulated by gradually leading nitrogen (99.999%) into the device. After hypoxia treatment for 6, 12 and 24 hours, the embryos received reperfusion for 6 hours under normal oxygen concentration. The embryos in the control group received normoventilation (the dissolved oxygen concentration was about 7.0 mg/L). Acridine orange staining was performed to observe the effect of different hypoxia durations on the apoptosis of neurons in zebrafish, and then the c-fos gene expression was quantitative analyzed with real-time quantitative nucleic acid amplification detection system. And the expression level of c-fos gene was compared before and after hypoxia reperfusion.
RESULTS AND CONCLUSION: A small amount of apoptotic brain cells could be detected in the control group, and the c-fos gene expression level was decreased; in the experimental group, the number of apoptotic cells was increased after hypoxia for 6, 12 and 24 hours, and the gene expression after hypoxia for 6 hours was increased distinctly. The results indicate that hypoxia can increase the c-fos gene expression in brain cells of zebrafish embryos, which may be one of the mechanisms of brain cell apoptosis increasing after hypoxia.

Key words: genes, fos, apoptosis, anoxia, reperfusion, zebrafish

CLC Number:

R318

Chen Yan-chen, Zhao Dan, Qing Di, Cheng Dong-liang, Mao Jiao-yu, Wang Bin. Zebrafish embryonic brain cell apoptosis and c-fos gene expression after hypoxia reperfusion[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(37): 6613-6619.

Figures/Tables 3

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