Repairing distal femur defects in rabbits with beta-calcium phosphate sintered bone

doi:10.3969/j.issn.2095-4344.2013.03.004

Abstract

Abstract:

BACKGROUND: Autologus bones possess the bone guided activity and osteoinductive properties, becoming the gold standard for repair of bone defects. However, the limitation of resources in autogenous bone causes the research fellows to look for bone graft substitutes.
OBJECTIVE: To explore the effectiveness of repairing distal femur defects in rabbit with β-calcium phosphate sintered bone.
METHODS: A rabbit model of distal femur defects at diameter of 5 mm and depth of 12 mm was prepared. β-calcium phosphate sintered bone was implanted into the experimental side of the model, and nothing to the control side. Operation incision was observed and the pathological section was prepared to observe bone growth after implantation.
RESULTS AND CONCLUSION: The incision healed fine after the operation of implanting the test piece of β-calcium phosphate sintered bone into the defect site in the condyles of femur in rabbits; new boneformation could be observed around bone defects in the rabbits of experimental groups after 4 weeks of the operation, the quantity of the new bone increased gradually with the time prolonging, the center of the β-calcium phosphate sintered bone could be found new bone and the materials could be degraded gradually after 12 weeks of the operation. In the other hand, new bone formation could not be found around bone defects until 12 weeks of the operation in the control group. These findings indicate that β-calcium phosphate has a good osteogenesis capacity, and can serve as an excellent bone graft substitute.

Key words: biomaterials, tissue-engineered bone materials, beta-calcium phosphate, controlled study, sintered bone, bone defects, bone implantation, biodegradation, biomaterial photographs-containing paper

CLC Number:

R318

Hou Xi-jun, Wang Chun-hua, Li Lin, Lian Hong-xing, Li Yu-qiang, Zhang Lian-qing. Repairing distal femur defects in rabbits with beta-calcium phosphate sintered bone[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(3): 400-406.

Figures/Tables 4

2.1 实验动物的一般情况在整个动物手术及其后续的检查过程中，所有动物无死亡发生，实验组第2号和4号动物在手术后发生单侧膝关节脱位，与初始手术过程中经验不足有关，但基本不影响动物术后的进食及日常活动。实验组1只动物发生手术切口周围的感染，经换药清创并局部使用抗生素后愈合。术后2周所有动物切口已经基本愈合，缝合线自动脱落。 2.2 兔股骨远端缺损后4，8，12周实验材料修复骨组织的大体观察结果术后4周：实验侧材料与周边组织紧密结合，周边新生骨组织向材料的孔隙内长入，原有骨缺损部位界限仍清楚，植入材料无明显吸收变小，其中心部位也无软组织或骨组织长入。对照侧缺损周边部位见少量新生骨组织，中心部位被软组织替代。术后8周：实验侧材料周边组织进一步向材料内部生长，材料面积与4周时相比明显减小，骨缺损部分边界不清，但中心部位大部分仍被植入材料占据，边缘新生骨组织部分替代植入材料；对照侧新生骨组织略有增多，但缺损部位仍清晰可辨，缺损中心仍被软组织占据。术后12周：实验侧材料被周边新生骨组织严密包裹，材料与新生骨组织界限不清，但缺损部位仍残留少部分植入材料，新生骨组织长入材料中心部位；对照侧骨缺损部位仍清晰可辨，以纤维组织增生替代为主，新生骨组织未长入骨缺损中心部位，缺损部位无法自行被新生骨组织填充。 2.3 兔股骨远端缺损后4，8，12周实验材料修复骨组织的钼靶X射线照相结果实验侧：4周时可见植入材料密度明显高于周围正常骨组织，材料的外形轮廓清晰完整，材料与周围正常骨组织的界限清晰，材料内的多孔结构清晰可辨；8周时，材料密度略有降低，材料与周围正常骨组织的界限仍清晰可辨，材料内部无明显的新生骨组织长入，材料可视面积无明显缩小；12周时材料的密度进一步降低，接近正常骨组织的密度，材料与周围正常骨组织的界限模糊，植入材料部分被新生骨组织替代，材料内部可见新生骨组织长入，见图2。"

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