Abstract:

BACKGROUND: Curcumin can significantly reduce the bone resorption of osteoblasts, inhibit osteoclastogenesis and induce apoptosis in ostebclasts.
OBJECTIVE: To investigate the effects of different concentrations of curcumin on osteoblast differentiation of rat bone marrow mesenchymal stem cells.
METHODS: Rat bone marrow mesenchymal stem cells were isolated by adherent method. After subcultured to the third generation, osteogenic induction was performed with osteogenic culture medium. 0 (control), 10 and 15 μmol/L curcumin was added into the osteogenic medium at 3 days before induction.
RESULTS AND CONCLUSION: After seeded and cultured for 7 days, scattered small colonies of bone marrow mesenchymal stem cells could be seen under the inverted microscope, and the colonies were in radial growth. As the culture time prolonged, the cell colonies were increased and the morphology of the cells was spindle shaped. At 7 days after addition of osteoblast medium, cells changed from spindle-shaped to cuboid and were more apparent by days. After addition of different concentrations of curcumin, there was no difference in the proliferation of bone marrow mesenchymal stem cells. Curcumin could increase the activity of alkaline phosphatase in bone marrow mesenchymal stem cells and the expression of Runx-2 and heme monooxygenase 1 in a dose-dependent manner. These findings demonstrate that curcumin can promote osteogenic differentiation of rat bone marrow mesenchymal stem cells. The effect of curcumin on osteogenic differentiation of rat bone marrow mesenchymal stem cells is correlated with heme monooxygenase 1 expression.