Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (27): 5052-5056.doi: 10.3969/j.issn.2095-4344.2012.27.021

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Influence of secreted cytokines of bone marrow mesenchymal stem cells on the stability of rat peritoneal mast cell membrane

Gao Ming-min1, Liu Xiao-ping2, Yu Ye-jun1, Jia Yue-wei3, Wang Jia-hui1, Li Wei2   

  1. 1Department of Dermatology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266012, Shandong Province, China;
    2Department of Histology and Embryology, Medical College of Qingdao University, Qingdao 266012, Shandong Province, China;
    3 Oak Biological Development Co., Ltd., Qingdao 266012, Shandong Province, China
  • Received:2011-12-02 Revised:2011-12-30 Online:2012-07-01 Published:2013-11-01
  • Contact: Yu Ye-jun, Master, Chief physician, Department of Dermatology, the Affiliated Hospital of Qingdao University Medical College, Qingdao 266012, Shandong Province, China

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Abstract:

BACKGROUND: Mesenchymal stem cells secreted cytokines has immunomodulatory effects on specific and nonspecific immune cells. Whether the cytokines have an effect on mast cells requires further studies.
OBJECTIVE: To explore the influence of the cytokines secreted by bone marrow mesenchymal stem cells on the degranulation of specific and nonspecific induced mast cells and secretion of inflammatory mediators.
METHODS: Rat bone marrow mesenchymal stem cells were isolated and purified by whole bone marrow adherent method. The stem cell cytokines were obtained from the supernatants of the third generation of bone marrow mesenchymal stem cells. The mast cells were isolated from rat peritoneal mast cells. Mast cells were specifically sensitized by rat antiovalbumin serum and nonspecifically stimulated by Compound48/80.
RESULTS AND CONCLUSION: Compared with the control group, cytokines from bone marrow mesenchymal stem cells and ketotifen could significantly inhibit the degranulation of the specific and nonspecific mast cells and release tryptase, and there was significant difference (P < 0.05), however, the effect of cytokines from bone marrow mesenchymal stem cells was weaker than that from the ketotifen (P < 0.05). Cytokines from bone marrow mesenchymal stem cells could stable mast cells membrane, inhibit the degranulation of mast cells and the release inflammatory mediators in mast cells.

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