Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4247-4252.doi: 10.3969/j.issn.1673-8225.2012.23.014

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Differentiation of bone marrow-derived neural stem cells transfected by nuclear receptor-related factor 1 gene into dopaminergic neurons in vitro

Liang Wei, Xu Zhi-en, Ke Jun-long, Chen Jing-juan, Li Hua   

  1. Department of Neurology, First Affiliated Hospital of Guangdong Medical College, Zhanjiang 523024, Guangdong Province, China
  • Received:2011-11-09 Revised:2012-01-18 Online:2012-06-03 Published:2013-11-06
  • Contact: Xu Zhi-en, Chief physician, Department of Neurology, First Affiliated Hospital of Guangdong Medical College, Zhanjiang 523024, Guangdong Province, China zjxuzhien@126.com
  • About author:Liang Wei★, Studying for master’s degree, Department of Neurology, First Affiliated Hospital of Guangdong Medical College, Zhanjiang 523024, Guangdong Province, China chinagdweiwei@qq.com

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Abstract:

BACKGROUND: Few reports have addressed whether genetic engineering of bone marrow-derived neural stem cells transfected by nuclear receptor-related factor 1 (Nurr1) gene can enhance the differentiation into dopaminergic neurons.
OBJECTIVE: To investigate the differentiation of bone marrow-derived neural stem cells transfected by Nurr1 gene into DA neurons in vitro.
METHODS: Rat bone marrow mesenchymal stem cells were in vitro cultured and purified and then divided into four groups. The un-transfected bone marrow-derived mesenchymal stem cells were divided into control group and brain-derived neurotrophic factor (BDNF) group; the screened recombinant plasmid-positive neural stem cells were divided into Nurr1 group and Nurr1+BDNF group.
RESULTS AND CONCLUSION: Plenty of Nurr1 in transfected bone marrow-derived mesenchymal stem cells were detected by RT-PCR after 4 days. Differentiation results showed that the mRNA expression of tyrosine hydroxylase in the cells of Nurr1+BDNF group was highest. The proportion of tyrosine hydroxylase positive cells in BDNF group, Nurr1 group and Nurr1+BDNF group was significantly higher than that in the control group after adherent differentiation of neural stem cells. The proportion of tyrosine hydroxylase-positive cells in the Nurr1+BDNF group was highest; the proportion was (52.44±15.9) %. The results indicate that the Nurr1 gene can promote bone marrow-derived mesenchymal stem cells to differentiate into dopaminergic neurons, and a large number of dopaminergic neurons are obtained after being induced by BDNF.

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