Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (23): 4227-4231.doi: 10.3969/j.issn.1673-8225.2012.23.010

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In vitro induced differentiation of adipose stem cells into dopaminergic neurons

Gao Hua1, 2, Yao Ya-ni1, Wang Yu-ling1, Yang Xin-ling1   

  1. 1Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China;
    2Department of Neurology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2011-09-24 Revised:2011-10-27 Online:2012-06-03 Published:2013-11-06
  • Contact: Yang Xin-ling, M.D., Chief physician, Professor, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China poplar862@sohu.com
  • About author:Gao Hua★, Master, Physician, Center of Neurological Medicine, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Department of Neurology, the Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China gaohuasnow@163.com

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Abstract:

BACKGROUND: Adipose stem cells are considered as the seed cells for the treatment of Parkinson’s disease due to their characteristics such as rich sources, simple drawing, easy to purify and amplify, autologous transplantation and multipotential differentiation.
OBJECTIVE: To observe the differentiation of rat adipose stem cells into dopaminergic neurons and their secreted function.
METHODS: The adipose stem cells of Sprague -Dawley rats were obtained by collagenase digestion. In the experimental group, the cells were differentiated into dopaminergic neurons co-induced by basic fibroblast growth factor, epidermal growth factor and ascorbic acid after cultured to the third passage in vitro. In the control group, the cells were simply added with 5% serum.
RESULTS AND CONCLUSION: Tyrosine hydroxylase expression in the experimental group was examined by immunofluorescence and immunohistochemisty after induced for 3 days. The proportion of dopaminergic neurons in experimental group was significantly higher than that in the control group (P < 0.01). The level of dopaminergic neurons in cell supernatant was detected by ELISA assay after successively induced for 3 days; the concentration of dopaminergic neurons was increased at the first and second days after induction, and decreased at the third day. The results showed that the adipose stem cells could be differentiated into dopaminergic neurons.

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