Abstract:

BACKGROUND: Thermosensitive chitosan shows good compatibility to many cells. It is a good vector in the tissue-engineering field. However, its effect on osteoblasts remains unclear.
OBJECTIVE: To study the cytotoxicity of chitosan thermosensitive hydrogel to osteoblasts.
METHODS: Osteoblasts were cultured in chitosan thermosensitive hydrogel, and then viewed cell morphology under a microscope; at the same time, osteoblasts were cultured with different concentrations of chitosan thermosensitive extracts in vitro for 24, 48, 72, and 96 hours, respectively. The MTT assay was used to determine the relative growth rate (RGR) of osteoblasts and determined the level of cytotoxicity.
RESULTS AND CONCLUSION: Osteoblasts were round at 24 hours after culture in chitosan thermosensitive hydrogel under a microscope, which extruded its horns and amplified after 48 hours. The RGF of osteoblasts cultured in different concentrations of chitosan thermosensitive extracts in vitro in each group was 92% to 112% at different time points, and the cytotoxic of all kind of concentrations of chitosan thermosensitive extracts were 0 or 1, which completely meet the standards of safety evaluation of biological materials.