Changes of mitochondrial respiratory function and PHB1
expression in rat skeletal muscle after moderate-intensity training

doi:10.3969/j.issn.2095-4344.2477

Abstract

Abstract:

BACKGROUND: Studies have shown that long-term moderate-intensity regular exercise can improve the activity of mitochondrial respiratory chain complex enzymes in skeletal muscle cells, thereby improving their power capability as well as the ability to resist fatigue.

OBJECTIVE: To explore the effect of moderate-intensity training on prohibitin (PHB) expression and mitochondrial respiratory function in rat skeletal muscle.

METHODS: Thirty-two healthy male Sprague-Dawley rats were randomly divided into two groups: quiet control group and moderate-intensity training group, each of 16 rats. In the moderate-intensity training group, the treadmill training was performed at a slope of 10°: in the 1^st week, running at 10 m/min, 10 minutes per day, 6 days per week; in the 2^nd week, running at 15 m/min, increased from 10 minutes per day to 60 minutes per day with an increase of 10 minute per day, 6 days per week; in the 3^rd to 8^th week, running at 15 m/min, 60 minutes per day, 6 days per week for a total of 8 weeks. Rats were sacrificed at 48 hours after the final experiment, and mitochondria were extracted from skeletal muscle samples. Mitochondrial respiration control rate, adenosine triphosphate content, reactive oxygen species level, complex V activity and PHB1 protein expression were measured.

RESULTS AND CONCLUSION: Compared with the quiet control group, in the moderate-intensity training group, mitochondrial respiration control rate in the skeletal muscle increased significantly (P < 0.001), adenosine triphosphate content increased significantly (P < 0.05), reactive oxygen species level decreased significantly (P < 0.001), the activity of complex V was significantly increased (P < 0.05) and PHB1 expression was significantly increased (P < 0.01). The correlation analysis showed that after 8 weeks of moderate-intensity training, the expression of PHB1 in the skeletal muscle of rats was positively correlated with adenosine triphosphate content and complex V activity, and negatively correlated with reactive oxygen species level. Therefore, moderate-intensity training improves mitochondrial oxidative phosphorylation, effectively maintains mitochondrial membrane structure and enhances mitochondrial respiratory function by increasing the expression of PHB1.

Key words: moderate-intensity training, skeletal muscle, mitochondrial respiratory function, PHB1, energy metabolism

CLC Number:

Fang Wen, Li Ze, Liu Zhaoming, Feng Hong. Changes of mitochondrial respiratory function and PHB1 expression in rat skeletal muscle after moderate-intensity training[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(8): 1207-1212.

Figures/Tables 4

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