Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (14): 2137-2141.doi: 10.3969/j.issn.2095-4344.3091

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Effect of high-intensity interval training on fat reduction and irisin expression in obese rats

Shi Xiaoyu1, 2, Feng Ziyang2, Liu Ziming3, Li Lin4, Wang Zhen2, Liu Xiaoran5, Yu Liang2   

  1. 1Department of Sports and Health, Handan Polytechnic College, Hebei Province, China; 2School of Sports and Human Sciences, Beijing Sport University, China; 3Tennis Management Center of the General Administration of Sports of China, China; 4Jiuzhou Polytechnic College, Jiangsu Province, China; 5Capital University of Physical Education and Sports, China
  • Received:2020-04-24 Revised:2020-04-26 Accepted:2020-05-27 Online:2021-05-18 Published:2020-12-30
  • Contact: Yu Liang, MD, Associate professor, Doctoral supervisor, School of Sports and Human Sciences, Beijing Sport University, Beijing 100084, China
  • About author:Shi Xiaoyu, Master, Lecturer, Department of Sports and Health, Handan Polytechnic College, Handan 056001, Hebei Province, China; School of Sports and Human Sciences, Beijing Sport University, Beijing 100084, China
  • Supported by:
    the National Natural Science Foundation of China, No. 31500964 (to YL)

Abstract: BACKGROUND: Studies have shown that high-intensity interval training is more time-saving than medium-intensity endurance training, which can effectively reduce visceral fat content.
OBJECTIVE: To observe the changes of body fat, fibronectin type III domain containing protein 5 (FNDC5) expression of the soleus muscle and serum irisin level after high-intensity interval training in obese rats. 
METHODS: Eighty healthy male Sprague-Dawley rats, 5 weeks old, were randomly divided into normal control group (n=20) and obesity group (n=60). The 30 rats were selected from the successful models and randomized into obesity quiet control group, moderate intensity exercise group and high-intensity intermittent training group (n=10 in each group), raising for 8 weeks. The change of the rat body mass was recorded, and samples were collected at quiet state after final exercise or at 12 hours after exercise. Body mass and body fat of the rats were measured, and the expression of FNDC5 in the soleus muscle was detected by immunoblotting, and the serum irisin level was determined. The experimental plan was approved by the Animal Experimental Ethics Committee of Beijing Sport University.
RESULTS AND CONCLUSION: Compared with the obesity quiet control group, the body mass of rats decreased (P < 0.01), body fat composition decreased (P < 0.05), FNDC5 protein expression increased (P < 0.01), and serum irisin level increased (P < 0.01) in the two obese exercise groups. Compared with the moderate-intensity exercise group, the body mass of rats was significantly lowered (P < 0.01), the body fat composition was decreased (P < 0.05), and the protein expression level of FNDC5 and the serum irisin levels were significantly increased in the high-intensity interval training group (P < 0.01). To conclude, exercise training can reduce body fat in rats by increasing the serum expression of irisin and FNDC5. The body fat content of rats in the high-intensity interval training group was lower than that in the moderate-intensity training group, and the serum irisin content and soles muscle FNDC5 protein expression were higher, indicating that the high-intensity interval training has a better effect on fat reduction in obese rats. 

Key words: skeletal muscle, high-intensity interval training, irisin, exercise, fat reduction, body fat, protein, model, rat

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