In vitro properties of magnesium doped hydroxyapatite coating on the surface of carbon/carbon composites

doi:10.3969/j.issn.2095-4344.2017.34.006

Abstract

Abstract:

BACKGROUND: In the early study, hydroxyapatite coatings containing magnesium (Mg-HA) were successfully prepared on the carbon/carbon composite surface by electromagnetic induction deposition. The effects of different induction deposition time on the morphology of Mg-HA coating were analyzed.

OBJECTIVE: To investigate the in vitro properties of Mg-HA coating on the carbon/carbon composites.

METHODS: HA (control group) and Mg-HA (experimental group) coating were prepared on the surface of carbon/carbon composites. (1) In vitro solubility: Carbon/carbon composites with different coatings were immersed in low-glucose DMEM medium, and surface morphology, ion concentration, and coating adhesion were tested before and after immersion. (2) Cytocompatibility: Carbon/carbon composites with different coatings were respectively cultured with mouse bone marrow mesenchymal stem cells for 6 days, and cell proliferation was measured by scanning electron microscope.

RESULTS AND CONCLUSION: (1) One day after soaking, sediments formed on the coating surface in both groups, but there were more sediments in the experimental group than the control group. Six days after soaking, the sediments completely covered the coating surface in the experimental group, while there were still some gaps in the control group; Ca and P were both detected on the coating surface in the two groups, and the Mg content on the coating surface was obviously higher in the experimental group than the control group. (2) Six days after soaking, the concentrations of Ca, P and Mg in the control group continued to fall; in the experimental group, the concentrations of Ca and P were also reduced gradually, but the Mg concentration arose gradually. Moreover, the Mg concentration was significantly higher in the experimental group than the control group (P < 0.05). (3) The bonding strength of the coating in the experimental group was higher than that in the control group before and after 6 days soaking. In summary, the Mg-HA coating has better bonding strength and cell compatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydroxyapatites, Magnesium, Tissue Engineering

CLC Number:

R318

Ni Xin-ye, Xiong Xin-bo,You Rui-jin, Wu Xing-sheng, Li Wan-shuai.

In vitro properties of magnesium doped hydroxyapatite coating on the surface of carbon/carbon composites

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(34): 5443-5448.

Figures/Tables 9

References

[1]Xin-ye N,Xiao-bin T,Chang-ran G,et al.The Prospect of Carbon Fiber Implants in Radiotherapy. J Appl Clin Med Phys.2012;13(4):3821.

[2]Zhou H,Hou S,Zhang M,et al.Deposition of calcium phosphate coatings using condensed phosphates (P2O74− and P3O105−) as phosphate source through induction heating.Mater Sci Eng C Mater Biol Appl.2016;69:337-342

[3]杨晓玲,曹俊杰,杨建,等.C/C复合材料表面聚多巴胺涂层的制备及性能研究[J].军事医学,2016,40(12):941-945,963.

[4]Ni XE,Tang B,Lin T,et al.Properties of plasma-spray coated hydroxyapatite on carbon/carbon composites pretreated by an argon plasma.New Carbon Mater.2013;28(3):215-221.

[5]Zhong P,Ni XE, Miao YL,et al.Individual humeral head replacement by C/C composite implants coated with hydroxyapatite via rotation plasma spraying.Sci Eng Compos Mater. 2015;22(3):325-330.

[6]倪昕晔,李爱军,白瑞成,等.掺镁羟基磷灰石涂层在碳/碳复合材料表面的制备[J].材料研究学报,2015,29(11):853-859.

[7]Crespi R,Mariani E,Benasciutti E,et al.Magnesium-Enriched Hydroxyapatite Versus Autologous Bone in Maxillary Sinus Grafting: Combining Histomorphometry With Osteoblast Gene Expression Profiles Ex Vivo.J Periodontol. 2009;80(4): 586-593.

[8]Nabiyouni M,Ren Y,Bhaduri SB.Magnesium substitution in the structure of orthopedic nanoparticles: A comparison between amorphous magnesium phosphates, calcium magnesium phosphates, and hydroxyapatites. Mater Sci Eng C Mater Biol Appl.2015;52:11-17.

[9]Park JW,Kim YJ,Jang JH,et al.Osteoblast response to magnesium ion-incorporated nanoporous titanium oxide surfaces.Clin Oral Implants Res.2010;21(11):1278-1287.

[10]Park JW,An CH,JeongSH,et al.Osseointegration of commercial microstructured titanium implants incorporating magnesium: a histomorphometric study in rabbit cancellous bone.Clin Oral Implants Res. 2012;23(3):294-300.

[11]周欢,杨蒙蒙,侯赛赛,等.掺杂不同离子的羟基磷灰石涂层研究现状[J].国际生物医学工程杂志, 2016,39(5):319-322.

[12]Su Y,Li D,Su Y.Improvement of the Biodegradation Property and Biomineralization Ability of Magnesium-Hydroxyapatite Composites with Dicalcium Phosphate Dihydrate and Hydroxyapatite Coatings. ACS Biomater Sci Eng. 2016;2(5): 818-828.

[13]Khalajabadi SZ,Ahmad N,Yahya A,et al.The role of titania on the microstructure, biocorrosion and mechanical properties of Mg/HA-based nanocomposites for potential application in bone repair. Ceram Int.2016;42(16):18223-18237.

[14]Ni XY,Li AJ,Xiong XB,et al.Effect of electromagnetic induction deposition's heating time of magnesium-hydroxyapatite coating on carbon/carbon composites.J Biobased Mater Bio. 2014;8(6):603-609.

[15]Xiong XB,Ni XY,Chu CC,et al.Effect of Induction Frequency of Chemical Liquid Vaporization Deposition on Preparation of Hydroxyapatite Coating on C/C composites.J Biobased Mater Bio.2015;9(5):537-543.

[16]Hench LL.Sol-gel materials for bioceramic applications.Curr Opin Solid St M.1997;2:604-610.

[17]LeGeros RZ.Properties of osteoconductive biomaterials: calcium phosphates. Clin Orthop Relat Res. 2002; 395:81-98.

[18]Xin-Bo X,Xin-Ye N,Ya-Yun L,et al.A Novel Strategy for Preparation of Si-HA Coatings on C/C Composites by Chemical Liquid Vaporization Deposition/Hydrothermal Treatments. Sci Rep.2016;6:31309.

[19]陈海波,憨勇,徐可为.等离子喷涂-水热合成羟基磷灰石涂层的体外稳定性[J].稀有金属材料与工程, 2005,34(5):754-757.

[20]熊信柏,邹春莉,冯岩鹏,等.钛合金表面高频感应热梯度法制备生物活性钙磷涂层探索[J].稀有金属材料与工程, 2007,36(7): 1249-1252.

[21]周宏明,曾麟,易丹青,等.电泳沉积制备BG/BG—FHA复合涂层[J].复合材料学报,2011,28(6):194-199.

[22]Zhao SF,Jiang QH,Peel S,et al.Effects of magnesium-substituted nanohydroxyapatite coating on implant osseointegration.Clin Oral Implants Res. 2013;24 (A100):34-41.

[23]Holmes D.Bone: Neuronal origin of osteogenic effects of magnesium.Nat Rev Endocrinol. 2016;12(12):687.

[24]Nakano Y,Le MH,Abduweli D,et al.A Critical Role of TRPM7 As an Ion Channel Protein in Mediating the Mineralization of the Craniofacial Hard Tissues.Front Physiol.2016;7:258.

[25]Abed E,Martineau C,Moreau R.Role of melastatin transient receptor potential 7 channels in the osteoblastic differentiation of murine MC3T3 cells.Calcif Tissue Int. 2011;88(3):246-253.

[26]Abed E,Moreau R.Importance of melastatin-like transient receptor potential 7 and magnesium in the stimulation of osteoblast proliferation and migration by platelet-derived growth factor.Am J Physiol Cell Physiol. 2009;297(2): C360-C368.

[27]Wang CL,Xiao F,Wang CD,et al.Gremlin2 Suppression Increases the BMP-2-Induced Osteogenesis of Human Bone Marrow-Derived Mesenchymal Stem Cells Via the BMP-2/Smad/Runx2 Signaling Pathway.J Cell Biochem. 2017;118(2):286-297.

[28]Chatzinikolaidou M,Pontikoglou C,Terzaki K,et al. Recombinant human bone morphogenetic protein 2 (rhBMP-2) immobilized on laser-fabricated 3D scaffolds enhance osteogenesis.Colloids Surf B Biointerfaces. 2017;149:233-242.