Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (34): 5437-5442.doi: 10.3969/j.issn.2095-4344.2017.34.005
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Received:
2017-10-14
Online:
2017-12-08
Published:
2018-01-04
Contact:
Sun Guan-wen, Department of Traumatic Orthopedics, Inner Mongolia People’s Hospital, Hohhot 010017, Inner Mongolia Autonomous Region, China
About author:
Sun Guan-wen, M.D., Chief physician, Department of Traumatic Orthopedics, Inner Mongolia People’s Hospital, Hohhot 010017, Inner Mongolia Autonomous Region, China
Supported by:
CLC Number:
Sun Guan-wen, Wang Jian, Shi Bin, Jia Peng.
2.2 支架的亲水性 PLGA/羟基磷灰石支架吸水率只有(35.32±0.54)%,PLGA /羟基磷灰石/ BMP-2支架吸水率增加到(35.78±1.07)%。随着壳聚糖含量的增加,PLGA/羟基磷灰石/BMP-2/壳聚糖支架的吸水率呈增加趋势,分别为(37.64±0.96)%,(39.15±1.23)%,(40.28±0.59)%。提示壳聚糖提高了复合材料PLGA/羟基磷灰石/BMP-2支架的亲水性。 2.3 各支架BMP-2的释放 使用ELISA试剂盒,测定60 d内支架释放BMP-2(图3)。加载BMP-2的支架在第1天呈爆发释放,随之呈持续缓慢释放。20 d内,支架表现出突发释放和递减释放。各支架BMP-2的累计释放的顺序:PLGA/羟基磷灰石/BMP-2支架35% > PLGA/羟基磷灰石/BMP-2/壳聚糖(0.25%)支架30% > PLGA/羟基磷灰石/BMP-2/壳聚糖(0.5%)支架28% > PLGA/羟基磷灰石/BMP-2/壳聚糖(1%)支架27%。在第20-24天,各支架释放BMP-2相同。但是,BMP-2累计从PLGA/羟基磷灰石/BMP-2/壳聚糖(1%)支架的释放接近PLGA/羟基磷灰石/BMP-2/壳聚糖(0.5%)支架。在第60天时,PLGA/羟基磷灰石/BMP-2、PLGA/羟基磷灰石/BMP-2/壳聚糖(0.25%)、PLGA/羟基磷灰石/ BMP-2/壳聚糖(0.5%)、PLGA/羟基磷灰石/BMP-2/壳聚糖(1%)支架BMP-2累积释放分别为44%、34%、27%和26%。这可能是支架表面附着壳聚糖抑制BMP-2释放。"
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