Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro

doi:10.3969/j.issn.2095-4344.2017.33.001

Abstract

Abstract:

BACKGROUND: Tetracycline hydrochloride is shown to promote viability and proliferation of human embryonic osteoblasts cultured in vitro, increase the production of type I collage and osteocalcin.
OBJECTIVE: To study the proliferation activity of bone marrow mesenchymal stem cells (BMSCs) cultured by tetracycline hydrochloride in vitro.
METHODS: Passage 3 BMSCs from Sprague-Dawley rats were cultured and divided into two groups: cells in control group were cultured in complete medium containing fetal bovine serum, and those in experimental group were cultured in complete medium containing tetracycline hydrochloride. After days 1-8 of culture, MTT and cell counting assay were used to detect cell viability and proliferation in the two groups.
RESULTS AND CONCLUSION: (1) Cell counting assay: with time, the number of cells in both groups was increased, but the cell number in the experimental group was higher than that in the control group at days 4-8 of culture (P < 0.05). (2) MTT assay: With time, the number of cells in both groups was increased: incubation period was at day 1, logarithmic growth period was within days 3-6, and plateau period began at day 7. Then, the cells continued to proliferate, and the cell proliferation was higher in the experimental group than the control group at days 5-8 of culture (P < 0.05). To conclude, tetracycline hydrochloride can significantly enhance BMSCs proliferation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Mesenchymal Stem Cells, Tetracycline Hydrochloride, Cell Proliferation, Tissue Engineering

CLC Number:

R394.2

Zhang Jue, Xue Si-liang, Luo Yuan, Zhi Wei. Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(33): 5249-5254.

Figures/Tables 2

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