Pillbug extracts inhibit osteopontin-induced vascular smooth muscle cell proliferation by modulation of integrin kinase protein pathway

doi:10.3969/j.issn.2095-4344.2017.16.021

Abstract

Abstract:

BACKGROUND: Pillbugs have been proved to exert an effect on the proliferation and migration of vascular
smooth muscle cells (VSMCs) through MTT assay and scratch assay, but the mechanisms are poorly understood.
OBJECTIVE: To investigate the molecular mechanisms underlying pillbugs influencing osteopontin-induced VSMC migration and proliferation.
METHODS: VSMCs from Sprague-Dawley rat thoraco-abdominal aorta were cultured in a common medium, and 3-5 generations of cells were selected. There were eight groups: control, osteopontin, pillbug water extract (0.5, 1.0, and 2.0 g/L) with osteopontin, and pillbug ethyl acetate extract (0.25, 0.5, and 1.0 g/L) with osteopontin groups. After 24 hours of culture, the expression levels of proliferating cell nuclear antigen (PCNA) and integrin kinase (ILK) were detected by western blot assay.
RESULTS AND CONCLUSION: Pillbug water and ethyl acetate extracts both inhibited the expression of PCNA, especially when cultured in 0.5 g/L pillbug water extract (77.8% of the osteopontin group) and 1.0 g/L ethyl acetate extract (74.1% of the osteopontin group). 0.5 g/L pillbug water extract significantly downregulated ILK level induced by osteopontin, which was 81.4% of the osteopontin group, while pillbug ethyl acetate extract exposed no influence on ILK expression. These results reveal that pillbug water and ethyl acetate extracts both are able to inhibit osteopontin-induced VSMC proliferation, and the role of the pillbug water extract maybe associated with ILK protein pathway.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Beetles, Osteopontin, Proliferating Cell Nuclear Antigen, Tissue Engineering

CLC Number:

R318

Duan Zhe-ping, Yu Xin-jiang, Li Jing-jing, Zhao Juan. Pillbug extracts inhibit osteopontin-induced vascular smooth muscle cell proliferation by modulation of integrin kinase protein pathway[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(16): 2582-2586.

Figures/Tables 1

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