Establishment and evaluation of a rabbit model of distraction osteogenesis

doi:10.3969/j.issn.2095-4344.2017.16.020

Abstract

Abstract:

BACKGROUND: Distraction osteogenesis is an effective method for large bone defects, bone tumors and osteomyelitis. However, there is a lack of a standard model in the basic research concerning distraction osteogenesis.
OBJECTIVE: To establish a rabbit femoral model of distraction osteogenesis and to assess its osteogenic effect.
METHODS: Twenty male New Zealand rabbits were enrolled to establish the rabbit femoral model of distraction osteogenesis using a novel distractor. Subsequently, the gross observation and X-ray examination of the specimens were performed to assess the osteogenic effect.
RESULTS AND CONCLUSION: Gross observation: on day 14 after fixation, there were light-colored and dense newborn bones distributing evenly in the distraction gap, and appeared with a columnar connection with the broken ends; the boundary with normal bones became vague. On day 35, the surface of newborn bones in the distraction gap showed the same color and texture with the normal ones, the boundary between the newborn and normal bones was difficult to distinguish, and the bone mineral density was increased notably. Radiology results: on day 14 after fixation, the stent was fixed stably, the broken ends got good reduction, and cloudy shallows connecting the two ends of the normal bones in the distraction gap and increased bone mineral density were detectable. Completely calcified new-born bones, intact bone cortex and open medullary canal were further visible on day 35. These results suggest that the rabbit femoral model of distraction osteogenesis is established successfully using the self-designed single-arm distractor based on rational surgical procedures and standard operations.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Models, Animal, Femur

CLC Number:

R318

Sun Bing-yin, Zhu Jie-ying, Xiong Jian-wei, Zhao Bao-xiang. Establishment and evaluation of a rabbit model of distraction osteogenesis[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(16): 2576-2581.

Figures/Tables 1

References

[1] Codivilla A. The classic: on the means of lengthening, in the lower limbs, the muscles and tissues which are shortened through deformity. 1905. Clin Orthop Relat Res. 2008; 466 (12):2903-2909.

[2] 孙青刚,康庆林,徐佳,等.Ilizarov外固定技术治疗重度足拇外翻疗效分析[J].国际骨科学杂志,2015,36(6):445-448.

[3] Xu J, Jia YC, Kang QL, et al. Management of hypertrophic nonunion with failure of internal fixation by distraction osteogenesis. Injury. 2015;46(10):2030-2035.

[4] 庞卫祥,许超,徐在强,等.补肾活血方在兔胫骨截骨延长过程中对TGF-β2基因表达的影响[J].浙江中西医结合杂志,2016,26(10): 902-905, 978.

[5] 任志勇,邱世超,黄现峰.骨形态发生蛋白2基因修饰自体骨髓间充质干细胞移植促进兔胫骨牵张成骨的实验研究[J].中国矫形外科杂志,2014,22(5):453-457.

[6] Hong P, Boyd D, Beyea SD, et al. Enhancement of bone consolidation in mandibular distraction osteogenesis: a contemporary review of experimental studies involving adjuvant therapies. J Plast Reconstr Aesthet Surg. 2013; 66(7):883-895.

[7] Xu H, Ke K, Zhang Z, et al. Effects of platelet-rich plasma and recombinant human bone morphogenetic protein-2 on suture distraction osteogenesis. J Craniofac Surg. 2013;24(2): 645-650.

[8] Malhotra A, Pelletier MH, Yu Y, et al. Can platelet-rich plasma (PRP) improve bone healing? A comparison between the theory and experimental outcomes. Arch Orthop Trauma Surg. 2013;133(2):153-165.

[9] Kim IS, Cho TH, Lee ZH, et al. Bone regeneration by transplantation of human mesenchymal stromal cells in a rabbit mandibular distraction osteogenesismodel. Tissue Eng Part A. 2013;19(1-2):66-78.

[10] Krishnan L, Willett NJ, Guldberg RE. Vascularization strategies for bone regeneration. Ann Biomed Eng. 2014; 42(2):432-444.

[11] Carlson EJ, Save AV, Slade JF 3rd, et al. Low-intensity pulsed ultrasound treatment for scaphoid fracture nonunions in adolescents. J Wrist Surg. 2015;4(2):115-120 .

[12] Salem KH, Schmelz A. Low-intensity pulsed ultrasound shortens the treatment time in tibial distraction osteogenesis. Int Orthop. 2014;38(7):1477-1482 .

[13] 黄鹤,李光早,朱永云.兔下颌骨牵张成骨实验模型的建立[J].蚌埠医学院学报,2009,34(7):555-557.

[14] 郑明,孙洪晨,刘春丽.兔双侧下颌骨牵张成骨牵张器的改进及动物模型的建立[J].吉林大学学报(医学版),2007,33(4):772-774, 779.

[15] 唐慧,王银龙,周健,等.羊下颌骨内置式三焦点牵张成骨的模型建立[J].安徽医科大学学报,2007,42(5):553-555.

[16] 曾景奇,黄枫,姜自伟,等.一种牵张成骨大鼠实验模型的建立[J].中国实验动物学报,2016,24(1)43-46.

[17] 柳玉晓,刘彦普,马芹,等.放射照射后牵张下颌骨成骨犬实验动物模型的建立[J].实用口腔医学杂志,2016,32(1)24-27.

[18] 刘冰,隋健夫,臧晓霞,等.失感觉神经支配大鼠下颌骨牵张成骨动物模型的建立[J].口腔颌面修复学杂志,2014,15(1)1-5.

[19] 19赵丹阳,姜闻博,张海峰,等.BMSCs联合3D打印PLLA支架促进兔颅骨PDO的研究[J].组织工程与重建外科杂志,2016, 12(6): 340-345, 352.

[20] 娄新田,房兵,沈国芳,等.牵张成骨区牙移动动物模型的建立[J].上海口腔医学,2011,20(1)21-25.

[21] 蔡鸣,沈国芳,林艳萍,等.基于快速原型技术的导航辅助下颌骨内置式牵张成骨术的实验研究[J].中国口腔颌面外科杂志, 2010, 8(5)427-435.

[22] 桂平,黄宇文,白植宝,等.兔下颌骨牵张成骨实验动物模型的建立[J].现代医院,2010,10(11)24-26.

[23] 黄若昆,林月秋,阮默,等.兔牵张成骨和骨段转移修复骨缺损模型的建立[J].中华实验外科杂志,2007,24(11):1442.

[24] 薛静,彭江,汪爱媛,等,活体小动物Micro-CT动态评价大鼠股骨牵张成骨[J].中国矫形外科杂志,2010,18(9):752-758.

[25] 李华,周诺.牵张成骨新生骨评价方法的研究及应用进展[J].山东医药,2012,52(14):94-96.

[26] Costantino PD, Friedman CD, Shindo ML, Experimental mandibular regrowth by distraction osteogenesis. Long-term results. Arch Otolaryngol Head Neck Surg. 1993;119(5): 511-516.

[27] 魏泓.医学实验动物学[M].成都:四川科学技术出版社,1998.

[28] 施新猷.现代医学实验动物学[M].北京:人民军医出版社,2000.

[29] Natu SS, Ali I, Alam S, et al. The biology of distraction osteogenesis for correction of mandibular and craniomaxillofacial defects: a review. Dent Res J (Isfahan). 2014;11(1):16-26.

[30] Alzahrani MM, Anam EA, Makhdom AM, et al. The effect of altering the mechanical loading environment on the expression of bone regenerating molecules in cases of distraction osteogenesis.Front Endocrinol (Lausanne). 2014;5:214.

[31] Peacock ZS, Tricomi BJ, Murphy BA, et al. Automated continuous distraction osteogenesis may allow faster distraction rates: a preliminary study. J Oral Maxillofac Surg. 2013;71(6):1073-1084 .

[32] Bright AS, Herzenberg JE, Paley D, et al. Preliminary experience with motorized distraction for tibial lengthening. Strategies Trauma Limb Reconstr. 2014;9(2):97-100.