Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (13): 2015-2022.doi: 10.3969/j.issn.2095-4344.2017.13.009

Previous Articles     Next Articles

Biological features and differentiation of human umbilical cord mesenchymal stem cells at different passages

Liu Yu-si1, 2, Wang Feng-chang1, Xia Hai-xiong1, 2, Xu Jian-wei1, Yang Yan1, Cui Dong-bing1, Wang Li-long3, He Zhi-xu1, 2, 3, Shu Li-ping1, 2, 4   

  1. 1Central for Tissue Engineering and Stem Cell Experiments, 2Department of Immunology, Basic Medical College, 3Medical Center for Children, 4Central for Experimental Center, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Revised:2016-12-23 Online:2017-05-08 Published:2017-06-09
  • Contact: Shu Li-ping, M.D., Associate professor, Central for Tissue Engineering and Stem Cell Experiments, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; He Zhi-xu, M.D., Professor, Central for Tissue Engineering and Stem Cell Experiments, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • About author:Liu Yu-si, Studying for master’s degree, Central for Tissue Engineering and Stem Cell Experiments, Guizhou Medical University, Guiyang 550004, Guizhou Province, China; Department of Immunology, Basic Medical College, Guizhou Medical University, Guiyang 550004, Guizhou Province, China
  • Supported by:

    the Major Special Scientific Project-“125 Plan” of Guizhou Province, No. [2013]020

Abstract:

BACKGROUND: As a kind of emerging seed cells in the field of tissue engineering and regenerative medicine, 
human umbilical cord mesenchymal stem cells are applied depending on whether it can remain stable biological characteristics and multi-directional differentiation capacity after long-term passage in vitro, which needs further experimental studies.
OBJECTIVE: To investigate differences of vital biological features of human umbilical cord mesenchymal stem cells over different passages. 
METHODS: Human umbilical cord mesenchymal stem cells taken from Wharton's jelly were isolated and cultured using tissue explant method. The proliferation abilities of cells cultured for 3-6 generations were detected using both cell counting method and MTT method. Expressions of surface markers of the cells after expanding over nine passages were comprehensively determined by flow cytometry. Karyotype analysis of cells cultured for 3-6 generations was assessed using cell staining method. The 3rd generation of cells were used to detect differentiation abilities into nerve cells, osteoblasts and hepatocytes.  
RESULTS AND CONCLUSION: Proliferation curve of cultured cells was “S” shape, and it was in the slow growth phase within 1-2 days, in the increased logarithmic growth phase within 2-4 days, in the plateau growth phase within 4-5 days, and in the declining growth phase within 5-6 days. Furthermore, two methods of testing results were consistent. Expression levels of cell surface markers CD90, CD44, CD73 were all above 90% while the expression levels of CD11b, CD18, CD34, CD43 and HLA-DR were very low at passages 1-9. No abnormality in cell karyotype was found in cells at passages 3-6. Human umbilical cord mesenchymal stem cells could differentiate into nerve cells, osteoblasts and hepatocytes. All theses experimental findings show that the human umbilical cord mesenchymal stem cells have high proliferation activity and multi-directional differentiation properties.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Umbilical Cord, Mesenchymal Stem Cells, Tissue Engineering

CLC Number: