Expression of stem cell surface marker CD133 in hepatocellular carcinoma and its positive subset proliferation

doi:10.3969/j.issn.2095-4344.2017.09.002

Abstract

Abstract:

BACKGROUND: CD133 has been used as a marker to separate and study different tumor stem cells by using its protein specific features.

OBJECTIVE: To investigate the expression of stem cell surface marker CD133 in hepatocellular carcinoma and the in vitro proliferation of its positive subsets.

METHODS: The human hepatoma MHCC97-H cell lines were cultured and sorted, CD133⁺and CD133^- MHCC97-H cells were obtained, and the expression of CD133 and cell migration and invasion were detected. After 7 days of culture, cell proliferation ability and Notch expression were detected. After 14 days of culture, cell cloning efficiency was detected. CD133⁺ and CD133^-MHCC97-H cells were implanted into nude mice subcutaneously, and 4 weeks later, tumor formation in mice was detected.

RESULTS AND CONCLUSION:(1) CD133 expression and cell clone formation: The expression of CD133 and cell cloning efficiency of CD133⁺MHCC97-H cells were significantly higher than those of CD133^-MHCC97-H cells (P < 0.05). (2) Cell proliferation: After 3-7 days of culture, the absorbance value of CD133⁺MHCC97-H cells was significantly higher than that of CD133^-MHCC97-H cells (P < 0.05). (3) Cell migration and invasion: The number of CD133⁺MHCC97-H cells passing through the cell membrane was significantly more than that of CD133^-MHCC97-H cells (P < 0.05). (4) The Notch protein expression of CD133⁺MHCC97-H cells was significantly higher than that of CD133^-MHCC97-H cells (P < 0.05). (6) Tumor formation test: The tumor size of CD133 ^-MHCC97-H cells was larger than that of CD133^-MHCC97-H cells (P < 0.05). To conclude, CD133⁺ hepatocellular carcinoma cell subsets with strong proliferation ability have some characteristics of cancer stem cells, and strong invasion, metastasis and tumorigenic abilities.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Neoplastic Stem Cells, Cell Proliferation, Tissue Engineering

CLC Number:

R394.2

Zhang Bao-qi. Expression of stem cell surface marker CD133 in hepatocellular carcinoma and its positive subset proliferation[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(9): 1319-1323.

Figures/Tables 4

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